[Histonet] bone tissue falling off after AR step

[Connolly, Brett M]

Hey boneheads,
A colleague is having problems with cortical bone detaching from the
slide during or after the antigen retrieval step. He is working with
mouse tibia. 

He uses Superfrost+ slides. The AR is with citrate buffer (pH6),
performed in a waterbath for 10 minutes. He bakes the slides for 15 min
at 56C prior to deparaffinizing.

What do you suggest?

Thanks, Brett

Brett M. Connolly, Ph.D.
Research Fellow
MRL, Imaging Research
Merck & Co., Inc.
WP-44K
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803
e-mail. brett_connolly <@t> merck.com


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