[Histonet] Von Kossa Stain

Nancy W. Troiano nancy.troiano <@t> yale.edu
Mon Dec 18 12:38:38 CST 2006

We use methyl green pyronin as a counterstain for our Von Kossa stain on 4 
-5 micron deplasticized sections of undecalcified bone embedded in MMA.  I 
don't know if that stain will work on thick undeplasticized sections, 
though.  Another possible counterstain that we use occasionally is 
toluidine blue, pH 3.7 which shows up the cells nicely.  

More information about the Histonet mailing list