[Histonet] hyaluronidase digestion for aggrecan staining

[Liz Chlipala]

Hello All

 

We have been working on aggrecan immunostain on articular cartiage and
wanted to perform a hyaluronidase digestion prior.  I have searched
extensively and have come up with few methods, none of which is complete.
Does anyone out there have a method that includes the molarity of the
buffer, the pH of the buffer and the amount of hyaluronidase used and the
length of time and temp, etc..  Any advice would be helpful.

 

Thanks in advance

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

P.O. Box 18592

Boulder, CO 80308

phone (303) 735-5001

fax (303) 735-3540

liz <@t> premierlab.com

www.premierlab.com

 

Ship to Address:

 

Premier Laboratory, LLC

University of Colorado at Boulder

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Boulder, CO 80309