[Histonet] Small endoscopy specimens

[Vicki Hakim]

hi 
i would like to also join my request for that protocol
we are currently trying to deal with that kind of a problem
and this is one of the few things that i haven't tried
thanks
vicki

----- Original Message -----
From: sheila adey <sheila_adey <@t> hotmail.com>
Date: Wednesday, August 30, 2006 17:33
Subject: Re: [Histonet] Small endoscopy specimens
To: rjbuesa <@t> yahoo.com, pkarlisch <@t> psu.edu, histonet <@t> lists.utsouthwestern.edu

> Hi,
> Do you use mineral oil on your processor for large specimens to? 
> I would 
> like to see your protocol aswell. We have on occasion had this 
> same problem.
> Thanks
> Sheila HT, MLT
> Port Huron Hospital
> 
> 
> 
> >From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> >To: Patricia Karlisch <pkarlisch <@t> psu.edu>, 
> >histonet <@t> lists.utsouthwestern.edu
> >Subject: Re: [Histonet] Small endoscopy specimens
> >Date: Tue, 29 Aug 2006 09:53:12 -0700 (PDT)
> >
> >Patricia:
> >   You yourself have pointed out to the 2 main 
> sources of the problem:
> >   1- a longer than needed processing schedule is 
> one. Reducing it, 
> >specially the dehydrating stations, can help, and
> >   2- doing a better coordination between the OR and 
> the lab (with regards 
> >to how expeditiously the Bx are fixed/delivered).
> >   In our case we did the same two things but we 
> really eliminated the 
> >problem when we eliminated xylene in our tissue processing and 
> changed to 
> >mineral oil.
> >   If you want I can send you that protocol.
> >   Regards
> >   Renי J.
> >
> >Patricia Karlisch <pkarlisch <@t> psu.edu> wrote:
> >   All,
> >We are having occassional problems with small colon, stomach and
> >antrum specimens that are 'drying' out, at least this is what 
> we think.
> >The specimens show no nuclear detail and the H&E looks smudgy. Other
> >specimens stained with the same slides look great. We process 
> our small
> >specimens on a short 5 hour processing schedule which seemed to have
> >improved some of our 85% of our specimens. Has anyone else had this
> >problem? We think that the specimen may be sitting in the OR before
> >they get placed in formalin. Is there any way to fix this when it
> >happens? Any suggestions would be helpful. Pat Karlisch
> >
> >Pat Karlisch
> >Supervisor, Histology, Pathology and Laboratory Medicine
> >Penn State Milton S. Hershey Medical Center
> >Mail Code H179
> >Hershey, PA 17033
> >Phone (717) 531-6072
> >Fax: (717) 531- 7741
> >email: pkarlisch <@t> psu.edu
> >
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