[Histonet] Mounting large cryosections

[Rene J Buesa]

Greg:
  Depending on what you are going to use the sections afterwards, Mayer's albumen is good (if dried on the selides before adding the cryosection).
  If you are going to use them for IHC, Mayer's will give you too much background.
  René J.

"Dasso, Greg (staff)" <dassog <@t> evergreen.edu> wrote:
  Histonetters,

I've been preparing CryoJane slides for my cryo sections on 25mm slides for the last year and they work great for 1 square-inch blocks. Our research has advanced and I am moving to a 4 square-inch machine that requires a 48mm slide. CryoJane makes their 2-inch tape for section collection, however they do not make a 48mm slide with the adhesive coating used on their 24mm slides. I had heard that albumin will work, but was wondering if anyone else is mounting larger, whole-animal cryo sections and what solutions you could offer.

thanks in advance,

Greg Dasso
Barlow Scientific, Inc
dassog <@t> evergreen.edu
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