[Histonet] contaminants in tissue processor

[Patsy Ruegg]

White blooms sounds like fungus.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim
O'Sullivan
Sent: Sunday, August 13, 2006 4:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] contaminants in tissue processor

Hi everyone,

We have a very old citadel tissue processor that we use to process mouse
kidney,liver and joints. We have found recently white blooms (best
description I can think of- looks like cotton wool) in the first pot of
histosol after the last alcohol- does anyone know what these could be and
how to prevent them?

Secondly since we have changed from using xylene to  histosol we seem to be
getting a toffee like substance on the bottom of the wax pots-is there any
way we can prevent this?

Any suggestions would be helpful


KimO'Sullivan

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