[Histonet] RE: sporadic CJD

[Richard Hessler, M.D.]

There is no reason not to pre-treat for the sake of safety. The following is from the NIH reccomendations:

Adequate fixation of small tissue samples (e.g. biopsies) from a patient with suspected prion disease is followed by post-fixation in 96% absolute formic acid for 30 minutes, followed by 48 hours in fresh 10% formalin. 

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Subject: Histonet Digest, Vol 33, Issue 16

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Today's Topics:

   1. FW: sporadic CJD (Team medizin.at)
   2. RE: Setting Ink (pruegg <@t> ihctech.net)


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Message: 1
Date: Sat, 12 Aug 2006 21:59:37 +0200
From: "Team medizin.at" <redaktion <@t> medizin.at>
Subject: [Histonet] FW: sporadic CJD
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<!~!UENERkVCMDkAAQACAAAAAAAAAAAAAAAAABgAAAAAAAAA+HZ2rsOEJ0CS6UEeZ3NhFMKAAAAQAAAAdyiCh2pRQke+boR6sbfWtwEAAAAA <@t> medizin.at>
	
Content-Type: text/plain;	charset="iso-8859-1"

Hi,

i´ve been asked to work up some formalin fixed heart, lung, liver etc. but NOT brain or CNS from a case with sporadic CJD. I´ve been told that this can be done without any precautions, like every other specimen.

Is this true? And if not true, how should the specimens be treated?

Please help and many thanks in advance!

Cristin




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Message: 2
Date: Sat, 12 Aug 2006 15:56:30 -0600
From: pruegg <@t> ihctech.net
Subject: RE: [Histonet] Setting Ink
To: "'Heckford, Karen - SMMC-SF'" <Karen.Heckford <@t> CHW.edu>,	"'Histonet
	\(E-mail\)'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200608122156.k7CLuXf1045204 <@t> pro12.abac.com>
Content-Type: text/plain;	charset="us-ascii"

We use Bouins fixative to set ink, just dip it in for a few seconds then go on your way.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF
Sent: Wednesday, August 09, 2006 8:04 AM
To: Histonet (E-mail)
Subject: [Histonet] Setting Ink

Cheers Everyone,  Does anyone know if there is something that sets the ink on tissue after you have inked the margins.  I vaguely remember something but I cannot seemed to dig it out of my brain.  
 
Thanks in advance,
Karen Heckford HT (ASCP) CE
Lead Histology Technician
Histology/Pathololgy Department
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
Fax: 415-750-8123
email: kheckfor <@t> chw.edu




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