[Histonet] Re: Setting Ink

pruegg <@t> ihctech.net pruegg <@t> ihctech.net
Sun Aug 13 18:17:32 CDT 2006

I just heard from someone suggesting vinegar (ta da acetic acid), so I think
you suggestion would work Bob.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
RSRICHMOND <@t> aol.com
Sent: Sunday, August 13, 2006 12:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Setting Ink

Karen Heckford asks >>Does anyone know if there is something that sets the 
on tissue after you have inked the margins?<< and Patsy Ruegg (along with 
several others) replies >>We use Bouin's fixative to set ink, just dip it in
a few seconds then go on your way.<<

Bouin's fixative contains picric acid, a potential explosion hazard that can

give you trouble with regulatory agencies, besides being toxic, and ruinous
clothing (particularly since it is inclined to eat through latex).

Acetone, also often recommended, is a fire and explosion hazard.

If 2% to 3% acetic acid works - and I haven't tried it personally - why not 
use that?

If you blot the specimen dry adequately, the ink will usually stick to it - 
India ink, Davidson and similar marking inks, and tattoo inks - I've used
all. Ink won't stick to cauterized surfaces (such as LEEP specimens), but
pathologist can see these margins under the microscope whether they're inked

or not.

Bob Richmond
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