[Histonet] antigen retrieval for IHC

[John Kiernan]

Maria, can you give chapter and verse for the Shi/Taylor
and Puchrler/Meloan papers? Most papers with Shi and Taylor
among the authors are about high temperature antigen retrieval
(boiling water, with pH optima for various antigens).
 
Holde Puchtler and Susan Meloan published many imortant papers
about staining ad histochemistry in the 1970s=1980s. Susan M 
was a histonetter in the 1990s. 

*****************
 
Maria Mejia wrote:
> 
> Hello,
> 
> I believe it was sometime in June of this year that there was a
> discussion on the
> histonet regarding "the simplest form" of reversing the effects for formalin
> fixation on tissue was to wash the tissue well (before) processing.
> 
> Well, I just read the article "Antigen retrieval IHC: Past, Present, &
> Future by
> Shan-Rong Shi, richard J. Cote & Clive R. Taylor (can google this
> article). It's
> a very interesting! Anyway, the article has a section titled non-heating
> AR method stating (this) same simple method by (Puchtler & Meloan). It also
> goes on to say that Elias JM (1990) adopted this method routinely by storing
> deparaffinized in fresh changes of 10% sucrose/PBS @ 4C overnight (before)
> IHC.
> 
> My questions are, does anyone know or tried this method used by Elias?
> And, can anyone explain the mechanism of 10% sucrose/PBS play in this
> AR method?
> 
> Just curious!
> 
> Maria Bartola Mejia
> Smith-Kettlewell Eye Research Institute
> San Francisco, CA 94115
> Email: maria <@t> ski.org
> Phone: (415)-345-2185
> 
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