[Histonet] spleen sectioning

Sharon Cooperman scoop <@t> mail.nih.gov
Thu Oct 13 15:05:17 CDT 2005

Dear Histonetites,

I have been asked to prepare some FFPE spleens that are cut "along 
the long axis".  (Apparently the standard cut for 
hematologists/pathologists to use?)   The way a spleen looks to me, 
there are three possible planes to cut it in;  one perpendicular to 
the longest dimension and two parallel to the longest dimension.  Of 
the the two planes parallel to the longest dimension, one would be 
parallel to the second longest dimension and one would be 
perpendicular to the second longest dimension.  I've been cutting my 
spleens parallel to the longest dimension and perpendicular to the 
second longest dimension, because that's where I seem to get the most 
information, and it also looks like what I see in my hematopathology 
textbook.  Is that correct?  I know there's probably a standard way 
to do it that everybody knows but I'm not a histologist, pathologist 
or hematologist and I'm pretty uninformed about the standard clinical 
ways to do spleens.  I would appreciate any advice, references, etc. 
Thanks in advance!

Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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