[Histonet] Immunoflouresence Questions

[Morken, Tim - Labvision]

Nita, 
Storage of flu0rochrome-labeled tissue slides in the fridge will retard
fading for up to a couple weeks, but if you look at the slides within a day
or so it is not really any better than room temperature storage in my
experience. Pictures should always be taken on first viewing to get the best
signal. 

A mounting medium containing DABCO (1,4-diazabicyclo[2.2.2]octane) will help
reduce fading. You can get it from Sigma, Cat# 10981

Tim Morken
Lab Vision - Neomarkers
www.labvision.com

Free webhosting for US State Histotechnology Societies:
http://www.labvisioncorp.com/demowebsite/index.cfm

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Thursday, October 13, 2005 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Immunoflouresence Questions


I have a tech that is asking these questions regarding possible "new
procedures". We have a new clinician that has "new" ideas.

1. Does storing slides in refrigerator after staining aid in anyway? (I had
never seen this before ; always stored in dark @ room temperature).

2. IS there a mounting media that aids in permanence?  

3. To my knowledge, these slides are not permanent (no matter what you
do)  but will inspectors really expect to see them- even in a faded
condition? (we take pictures of the kidneys)

Thanks

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