[Histonet] MOUSE BRAIN FIXATION

[Geoff McAuliffe]

Years ago my colleagues who worked on mouse bloood would withdraw up to 
1 ml of blood from the mouse heart in a similar manner, but hitting the 
left ventricle does take some practice.
I am a little amazed at the low volume of fixative as well, but tilting 
the animal so that the head is lower than the heart would help.
Note that the resolution of the photos is not all that good.

Geoff



Favara, Cynthia (NIH/NIAID) wrote:

>I have this paper and hope to try this next week. Note that the fixative is
>picric acid/paraformaldehyde/gluteraldehyde. I would love to get great
>fixation with 1ml and without opening the chest cavity. I will let you know!
>
>c
>
>Cynthia Favara
>NIAID/NIH/RML/LPVD
>903 South 4th Street
>Hamilton, MT 59840
>406-363-9317
>
>-----Original Message-----
>From: Adam Perry [mailto:kaleid11 <@t> yahoo.com] 
>Sent: Thursday, October 13, 2005 8:20 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] MOUSE BRAIN FIXATION
>
>I downloaded the technical report on this variant perfusion technique.  I'm
>amazed that you can get good fixation of the brain with 1 ml of fixative
>pumped through the heart without clamping off descending aorta, etc.  Has
>anyone tried this technique (or similar variants), and what kind of results
>were obtained?
> 
>I'm planning on trying it in a week or so, because I would love to get good
>brain fixation with so little time, fixative and waste production...but am
>wary...
> 
>Adam Perry
> 
>Department of Physiology and Biophysics
>University of Illinois 
>Chicago, IL 60612
> 
>There have been several inquiries regarding fixation of mouse brains
>recently.
> 
>A short technical report has just been published in BioTechniques:
> 
>Minimally invasive method for murine brain fixation
>Eichenbaum KD et al
>Biotechniques 2005; 39(4): 487-500
> 
>After simple registration, it can be accessed at
>http://www.biotechniques.com <http://www.biotechniques.com> 
>
>Ronnie Houston
>Director of Anatomic Pathology
>Bon Secours HealthPartners Laboratories
>5801 Bremo Road
>Richmond, VA 23226
>(804) 2877972
>(804) 2877906 - fax
>ronnie_houston <@t> bshsi.com
>
> 
>
>		
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-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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