[Histonet] MOUSE BRAIN FIXATION

[Favara, Cynthia (NIH/NIAID)]

I have this paper and hope to try this next week. Note that the fixative is
picric acid/paraformaldehyde/gluteraldehyde. I would love to get great
fixation with 1ml and without opening the chest cavity. I will let you know!

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Adam Perry [mailto:kaleid11 <@t> yahoo.com] 
Sent: Thursday, October 13, 2005 8:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] MOUSE BRAIN FIXATION

I downloaded the technical report on this variant perfusion technique.  I'm
amazed that you can get good fixation of the brain with 1 ml of fixative
pumped through the heart without clamping off descending aorta, etc.  Has
anyone tried this technique (or similar variants), and what kind of results
were obtained?
 
I'm planning on trying it in a week or so, because I would love to get good
brain fixation with so little time, fixative and waste production...but am
wary...
 
Adam Perry
 
Department of Physiology and Biophysics
University of Illinois 
Chicago, IL 60612
 
There have been several inquiries regarding fixation of mouse brains
recently.
 
A short technical report has just been published in BioTechniques:
 
Minimally invasive method for murine brain fixation
Eichenbaum KD et al
Biotechniques 2005; 39(4): 487-500
 
After simple registration, it can be accessed at
http://www.biotechniques.com <http://www.biotechniques.com> 

Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax
ronnie_houston <@t> bshsi.com

 

		
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