[Histonet] MOUSE BRAIN FIXATION

[Adam Perry]

I downloaded the technical report on this variant perfusion technique.  I'm amazed that you can get good fixation of the brain with 1 ml of fixative pumped through the heart without clamping off descending aorta, etc.  Has anyone tried this technique (or similar variants), and what kind of results were obtained?
 
I'm planning on trying it in a week or so, because I would love to get good brain fixation with so little time, fixative and waste production...but am wary...
 
Adam Perry
 
Department of Physiology and Biophysics
University of Illinois 
Chicago, IL 60612
 
There have been several inquiries regarding fixation of mouse brains
recently.
 
A short technical report has just been published in BioTechniques:
 
Minimally invasive method for murine brain fixation
Eichenbaum KD et al
Biotechniques 2005; 39(4): 487-500
 
After simple registration, it can be accessed at
http://www.biotechniques.com <http://www.biotechniques.com> 

Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax
ronnie_houston <@t> bshsi.com

 

		
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