[Histonet] H&E Staining of Frozen Sections

[gillian.2.brown <@t> gsk.com]

Any bets on the next question being 'what kind of haematoxylin'?

Gill Brown


T
GlaxoSmithKline Medicines Research Centre,
STEVENAGE,
Hertfordshire. UK
  SG1 2NY




"Rene J Buesa" <rjbuesa <@t> yahoo.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
17-Nov-2005 18:29
 
To
"Rebecca Jo" <beckyjo <@t> email.unc.edu>, "Histonet Listserv" 
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
Re: [Histonet] H&E Staining of Frozen Sections






We used to follow the following protocol:
  1- fix the frozen section  in acetone X 10 secs.
  2- distilled water x 10 secs
  3- hematoxylin x 30 secs
  4- tap water
  5- quick dip in acid alcohol
  6- eosin 5-10 secs.
  7- 95% ethanol > 100% ethanol x2 > xylene X2 > coverslip.
  The pathologists had the slide ready in about 2-3 minutes.
  Rene J.

Rebecca Jo <beckyjo <@t> email.unc.edu> wrote:
  Hi Histoneters-

I was wondering if someone could give me a protocol for H&E staining on 
frozen
sections. I assume I can just skip over the deparaffinizing steps and go
straight to the staining, but I wanted to know how long I should keep the
sections in hematoxylin. Just enough to stain the sections? Any help would
be greatly appreciated.

-Cheers

-- 
Rebecca E. Jo
UNC-School of Medicine
Department of Cell and Developmental Biology
(919)843-9648
CB# 7090

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