[Histonet] Recycled xylenes

[Osborn, Sharon]

Betsy,
	The recycled xylenes become more pure over time than that you
purchase commercially.  Xylenes is by definition, plural and a mixture of
ortho, meta and para isomers of xylenes with some ethyl benzene.  The
recycled works well in all functions of the histology laboratory except one.
If you are using the Sakura tape coverslipper, you will need to use some
newly purchased xylenes to run it.  The recycled, ie., "purified" xylenes do
not work as well with the tape coverslipper's adhesive causing it to not
activate to "stick tight".  I found this out through experience; the tape
coverslipper needs the impurities in the xylenes to do its job(as best as it
can). We solved this problem by having the last two stations of xylene on
the autostainer to be the commercial xylenes rather than the recycled
xylenes; or have two stations that you hand dip through before loading the
carrier on the tape slipper.
 There seems to be no problem with the mounting solutions used in glass
coverslippers by using the recycled xylenes.  Enjoy your savings of money
and the environment with your recyclers!
sharon osborn
DNAX, Schering-Plough BioPharma
Palo Alto, Ca



"Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> wrote:
  Hi,

I am interested in knowing if you have preferences for your recycled
xylenes. Do you use it just for staining, or processing alone. Or use it for
both?

Thank you.



Betsy Molinari HT (ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave.

Houston,TX 77030

832-355-6524

832-355-6812 (fax)



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