[Histonet] Cold acetone

[Bruijntjes, J.P.]

Steve

 

I've used cold acetone (-20) to fix tissues. First of all the acetone
was cooled down to 4 degrees in the refrigerator, the tissues were
collected and placed in the cold acetone, 15 minutes later the jar was
placed in a freezer (+ - 20 degrees) for another 2 hours. I used it to
fix and embed rat mammary glands using the AMEX method about 15 years
ago in order to do some immunostaining with all kind of different
monoclonal keratins (with good results).

 

Joost

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: woensdag 4 mei 2005 1:43
To: steven.coakley <@t> mirusbio.com; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Cold acetone

 

Steve,

the only "cold" acetone I used was just placing a coplin jar with
acetone in 

a refrigerator, which brought the temp down to + 4,. I've never used it 

at -20. What application are you using the cold acetone for?

 

Joe Nocito BS, HT(ASCP)QIHC

Histology Manager

Pathology Reference Lab

San Antonio, TX

----- Original Message ----- 

From: "Steven Coakley" <steven.coakley <@t> mirusbio.com>

To: <histonet <@t> lists.utsouthwestern.edu>

Sent: Wednesday, April 27, 2005 9:12 AM

Subject: [Histonet] Cold acetone

 

 

>

>

> I hope this doesn't sound to lame.  13 years in this field and I've
never

> used "cold acetone".  The only ref. I have is to use -20 cold acetone.
So

> how do I get my acetone to -20.  Do I place a coplin jar of covered 

> actenone

> in the cyostat for a period of time?????.  \\Steve

>

>

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