[Histonet] Cold acetone

[Joe Nocito]

Steve,
the only "cold" acetone I used was just placing a coplin jar with acetone in 
a refrigerator, which brought the temp down to + 4,. I've never used it 
at -20. What application are you using the cold acetone for?

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message ----- 
From: "Steven Coakley" <steven.coakley <@t> mirusbio.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 27, 2005 9:12 AM
Subject: [Histonet] Cold acetone


>
>
> I hope this doesn't sound to lame.  13 years in this field and I've never
> used "cold acetone".  The only ref. I have is to use -20 cold acetone.  So
> how do I get my acetone to -20.  Do I place a coplin jar of covered 
> actenone
> in the cyostat for a period of time?????.  \\Steve
>
>
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