[Histonet] RE: Ki67 - kidney

[C.M. van der Loos]

   Christian,

   You didn't tell us what rabbit Ki67antibody you purchased, but just in
   case it isn't working: we used rabbit monoclonal anti-Ki67, clone SP6
   from LabVision/Neomarkers successfully on mouse, rat and human FFPE
   tissue sections. Just a citrate6.0 or Tris-EDTA9.0 pretreatment and
   2-step EnVision detection.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389
   e-mail: [1]c.m.vanderloos <@t> amc.uva.nl


   ----- Original Message -----
                                       From 
   "christian <@t> eisbo.dk" <christian <@t> eisbo.dk>
                                       Date 
   Mon, 02 May 2005 10:31:07 +0200
                                         To 
   "histonet <@t> lists.utsouthwestern.edu"
   <histonet <@t> lists.utsouthwestern.edu>
                                    Subject 
   [Histonet] Ki67 - kidney.

   Hey users of histonet.
   I am trying to stain rat kidneys for Ki67!
   I have bought the recomended antibody. But this one is for human!
   Do anyone know if this can be used since i dont have a outcome!
   Do anyone have a protocol that works?
   Christian Eisbo

References

   1. mailto:c.m.vanderloos <@t> amc.uva.nl