[Histonet] Decals for IHC
Dorothy.L.Webb <@t> HealthPartners.Com
Dorothy.L.Webb <@t> HealthPartners.Com
Fri Mar 11 12:53:31 CST 2005
We use Rapid Cal Immuno from BBC for our decals and it works great and is
wonderful with the IHC's! It was a fairly recent switch and improved our
quality. They are very good at giving you a sample. Contact Adrain
Biesecker at 1-800-635-4477. Good luck!
-----Original Message-----
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Sent: Friday, March 11, 2005 12:10 PM
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Subject: Histonet Digest, Vol 16, Issue 21
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Today's Topics:
1. Fatty tissues processing (Stephen Peters M.D.)
2. Re: FDA statement for ASR antibodies used in
immunohistochemistry (Angela Bitting)
3. Placenta anyone? (Michael Titford)
4. Re: cell surface antigens (Gayle Callis)
5. Updating my address book (Kim Tournear)
6. NSH Self Assessment Series CD (kerry.l.crabb <@t> gsk.com)
7. Tau antibody (Jennifer Hofecker)
8. RE: IHC humidity chamber? (Jennifer MacDonald)
9. RE: FDA statement for ASR antibodies used
inimmunohistochemistry (Joe Nocito)
10. RE: IHC humidity chamber? (Gayle Callis)
11. Adipose tissue (Margaret Blount)
12. IHC with decal (Jesus Ellin)
13. Re: IHC with decal (clifford berger)
14. IHC Decal (Thomas Wood)
15. RE: IHC with decal (Carole Fields)
16. Question about markers for Graw Factor Antibody
(Lopez Manzano, Elisenda)
17. Reichert Histostat manual (Lett, Jaclynn)
18. Re: Placenta anyone? (Patti Loykasek)
19. Re: Reichert Histostat manual
(Don.Birgerson <@t> leica-microsystems.com)
20. Re: Tau antibody (Dana Settembre)
21. F4/80 on human tumour xenografts in mouse (ncragg)
22. RE: IHC with decal (Patsy Ruegg)
23. workshops (Robin Olmscheid)
24. Prion Lab (Kathleen Boozer)
25. exhausting tissue blocks (Dawn Cowie)
----------------------------------------------------------------------
Message: 1
Date: Thu, 10 Mar 2005 10:24:22 -0800 (PST)
From: "Stephen Peters M.D." <petepath <@t> yahoo.com>
Subject: [Histonet] Fatty tissues processing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050310182422.63542.qmail <@t> web30409.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi Dorothy,
I am sure you will responses from people who know a lot more about such
protocols than I do. I will give you a pathologists point of view. First,
whoever is doing your grossing has to cut these tissues no more than 3 mm
thick across the entire piece. If you are dealing with residents this is a
common problem. From the edge it looks 3 mm but at the center it is thicker.
I tell my gang to try for 2-3 mm with fatty tissues. Once cut the proper
thickness, it must fix in a proper quantity of formalin for more time than
it sits in the processor. I would make sure after cutting the 3mm section,
most fatty tissues spend at least a 3 hours more in formalin before putting
it in the processor. In the case of breast biopsies there is too much at
risk in trying to read a poorly processed section. We have gone to letting
most breast specimens fix overnight before processing. But it still has to
be cut thin prior to fixing or you will be wasting your time. Rather than
have special processing protocols for fatty tissue, you may find better
results by simplymaking sure your grossing people taking proper care in
sectioning and fixation of the tissues.
Stephen Peters MD
Pathology Innovations
------------------------------
Message: 2
Date: Thu, 10 Mar 2005 13:29:53 -0500
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] FDA statement for ASR antibodies used in
immunohistochemistry
To: <histonet <@t> lists.utsouthwestern.edu>, <Vickroy.Jim <@t> mhsil.com>
Message-ID: <s2304be4.090 <@t> GHSGWIANW2.GEISINGER.EDU>
Content-Type: text/plain; charset=US-ASCII
Univ. of Pittsburgh and Geisinger use our own names.
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
>>> "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com> 03/10/05 12:43 PM >>>
When using the FDA disclaimer in surgical reports when using an FDA
disclaimer are people using their own lab name in the disclaimer statement
(since we now have to validate the use of ASR's and RUO's), or are they
using the vendor's name of the company that developed the antibody, such as
Cell Marque, Ventana, BD, etc.
Jim Vickroy
Memorial Medical Center
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Message: 3
Date: Thu, 10 Mar 2005 10:36:45 -0600
From: "Michael Titford" <mtitford <@t> usouthal.edu>
Subject: [Histonet] Placenta anyone?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s230234b.054 <@t> gwia.usouthal.edu>
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Jennifer McDonald asks about placenta.
We routinely exam every placenta from every baby born at out Children's &
Woman's hospital. Normal placenta receive "gross only" treatment of weights,
dimensions and description. Abnormal history or findings trigger a "gross
in" of four blocks containing proximal, medial and distal cord, membranes,
full thickness and sample of any pathological areas found. Abnormal history
includes history of premature birth, abruption, history of more than one
spontaneous abortion, overweight baby, etc, etc. I think examining placenta
is now pretty much the standard of care. OBGYN people like documentation of
placenta from problem births in case a case goes to court
Mike Titford
USA Pathology
Mobile AL USA.
------------------------------
Message: 4
Date: Thu, 10 Mar 2005 11:59:27 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] cell surface antigens
To: "Till, Renee" <TillRenee <@t> uams.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050310115721.01b8f2c8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Renee,
For what species, human or murine?
At 10:05 AM 3/10/2005, you wrote:
>When staining for cell surface antigens, is there a difference between
>cyrosections or FFPE? Or would it depend on the particular antibody?
>Also, with antigen retrieval, have you noticed any difference between
>solutions and pH?
>
>
>
>Renee'
>
>
>Confidentiality Notice: This e-mail message, including any attachments,
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 5
Date: Thu, 10 Mar 2005 19:29:17 +0000 (GMT)
From: Kim Tournear <ktournear <@t> cox.net>
Subject: [Histonet] Updating my address book
To: histonet <@t> pathology.swmed.edu
Message-ID: <14830568.1110482957989.JavaMail.Administrator <@t> win01>
Content-Type: text/plain; charset=us-ascii
Hi
I am creating an address book that keeps all my contacts updated. I would
like to include you in it. Please use the link below to enter your details
for me:
http://www.bebo.com/fr1/10343558a511515716b145692819c478583130d18
We will exchange our contact information and every time we make changes they
will automatically update in our address books.
Thank You!
Kim
------------------------------
Message: 6
Date: Thu, 10 Mar 2005 15:07:02 -0500
From: kerry.l.crabb <@t> gsk.com
Subject: [Histonet] NSH Self Assessment Series CD
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF1627F607.8A6E7DBF-ON85256FC0.006D1483-85256FC0.006E7C45 <@t> glaxo.com>
Content-Type: text/plain; charset=us-ascii
NSH did have some defective CDs in the Self Assessment series. These were
found after selling them during the S/C in Toronto. Most if not all of
them have been replaced with a new copy. Additional problems have been
found with some of the new CDs. NSH is working with the vendor to
identify the source of these problems and how it will be fixed. If you
have a problem with your CD, please contact the NSH office via email at
"histo <@t> nsh.org". The NSH office with communicate with you about a fix
when the problem is resolved.
------------------------------
Message: 7
Date: Thu, 10 Mar 2005 12:14:09 -0800 (PST)
From: Jennifer Hofecker <jhofecker <@t> yahoo.com>
Subject: [Histonet] Tau antibody
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050310201409.37564.qmail <@t> web21002.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi everyone,
Are any of you currently using Tau from Dako? What
detection system are you using? I Would appreciate all positive/negative
input regarding this antibody.
Also, has another supplier's Tau proven better in your experience?
Thanks in advance,
Jennifer Hofecker
__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/
------------------------------
Message: 8
Date: Thu, 10 Mar 2005 13:05:37 -0800
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: RE: [Histonet] IHC humidity chamber?
To: Dana Settembre <settembr <@t> umdnj.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu,
JOHN.PHILLIPS <@t> new-tr.wales.nhs.uk,
histonet-bounces <@t> lists.utsouthwestern.edu, kosmicdog <@t> hotmail.com
Message-ID:
<OF9DFEA644.A0C98465-ON88256FC0.0073B674-88256FC0.00749234 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
Ted Pella has Immunostain Moisture Chambers. They hold 10 slides. We are
using them in our student IHC labs. They are working well for us. The
catalog number is 21049 and list price is $43.00. See www.tedpella.com
Jennifer MacDonald
------------------------------
Message: 9
Date: Thu, 10 Mar 2005 16:45:47 -0600
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] FDA statement for ASR antibodies used
inimmunohistochemistry
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPMEOICIAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain; charset="us-ascii"
Jim,
we just had a CAP inspection and the inspector was looking just for that
statement. We have our lab name in the disclaimer because we were the ones
that performed all the testing.
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim
Sent: Thursday, March 10, 2005 11:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FDA statement for ASR antibodies used
inimmunohistochemistry
When using the FDA disclaimer in surgical reports when using an FDA
disclaimer are people using their own lab name in the disclaimer statement
(since we now have to validate the use of ASR's and RUO's), or are they
using the vendor's name of the company that developed the antibody, such as
Cell Marque, Ventana, BD, etc.
Jim Vickroy
Memorial Medical Center
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 10 Mar 2005 16:15:04 -0700
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: RE: [Histonet] IHC humidity chamber?
To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050310161125.01b54a48 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
These are identical to the ones Evergreen Scientific makes and sells only
their prices are 3 boxes/pkg and you pay approx $80 for the pkg of 3 boxes,
considerably cheaper unless Evergreen has changed their pricing in recent
times.
I wonder if Evergreen is the manufacturer of these boxes for Ted
Pella? They look exactly the same.
02:05 PM 3/10/2005, you wrote:
>Ted Pella has Immunostain Moisture Chambers. They hold 10 slides. We
>are using them in our student IHC labs. They are working well for us.
>The catalog number is 21049 and list price is $43.00. See
>www.tedpella.com Jennifer MacDonald
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 11
Date: Fri, 11 Mar 2005 08:41:49 -0000
From: Margaret Blount <mab70 <@t> medschl.cam.ac.uk>
Subject: [Histonet] Adipose tissue
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2A70D44ECF6F1A4390DD1D98E8BEDEF211149F <@t> mius2.medlan.cam.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters,
Like Dorothy Webb, I too would like to receive advice about a good process
for adipose tissue. In my case, I need good sections of adipose tissue
itself. My process isn't too bad but I would like to improve it as I get
breaks in some the cell boundaries and would like to ensure the best
possible preparation. I am particularly interested in what clearing agents
are recommended and if anyone has a preference as to which make of wax they
like. I have to say that I am not keen on using xylene as clearing agent
since it seems to harden some of my other tissues too much. I would like to
use the same reagents for all my samples, even if I have to use different
timings for different batches of processing, I can set up to 10 programmes
on my processor (Leica TP1020, 12 stations, all with vacuum, ambient
temperature except for the wax stations of course.)
Thanks a lot.
Margaret
Margaret Blount
Chief Technician
Clinical Biochemistry
University of Cambridge
Addenbrooke's Hospital
Hills Road
Cambridge
CB2 2QR
------------------------------
Message: 12
Date: Fri, 11 Mar 2005 05:53:01 -0700
From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
Subject: [Histonet] IHC with decal
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <s2313254.093 <@t> FSGROUPWISE.yumaregional.org>
Content-Type: text/plain; charset=us-ascii
I am wondering what people are using for a decal solution that does not
interfere with IHC staining. ?? All the Help would greatly be
appreciated.
Jesus Ellin
Yuma Regional Medical Center
------------------------------
Message: 13
Date: Fri, 11 Mar 2005 08:25:09 -0500
From: clifford berger <miffordclark <@t> optonline.net>
Subject: Re: [Histonet] IHC with decal
To: Jesus Ellin <JEllin <@t> yumaregional.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <000601c5263d$bf499be0$0300a8c0 <@t> dellovo0ll7kuk>
Content-Type: text/plain; format=flowed; charset=iso-8859-1;
reply-type=original
Any of our decalicifiers work fine. You should take a look at our website,
www.decal-bone.com. The technical reprints and links page is filled with
articles about IHC after decalcification. You can also call us for free
samples.
Best regards,
Cliff Berger
Decal Chemical Corporation
1-800-428-5856
www.decal-bone.com
----- Original Message -----
From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, March 11, 2005 7:53 AM
Subject: [Histonet] IHC with decal
>I am wondering what people are using for a decal solution that does not
>interfere with IHC staining. ?? All the Help would greatly be
>appreciated.
>
> Jesus Ellin
> Yuma Regional Medical Center
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Fri, 11 Mar 2005 08:40:51 -0500
From: "Thomas Wood" <wood <@t> dcpah.msu.edu>
Subject: [Histonet] IHC Decal
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s23159a0.058 <@t> dcpah2.dcpah.msu.edu>
Content-Type: text/plain; charset=US-ASCII
We use Decal II from Surgipath and it does a descent job. RDO I have
little luck.
-Tom Wood
Michigan State University
------------------------------
Message: 15
Date: Fri, 11 Mar 2005 09:18:16 -0500
From: Carole Fields <cgfields <@t> lexhealth.org>
Subject: RE: [Histonet] IHC with decal
To: 'Jesus Ellin' <JEllin <@t> yumaregional.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CA70329E0BA39C4A974EDC102ADCE79E0BF63F20 <@t> mail.lmc.lexhealth.org>
Content-Type: text/plain; charset="iso-8859-1"
We use Rapid-Cal Immuno from BBC. It is working fine.
Carole Fields, HT,ASCP
Pathology Supervisor
Lexington Medical Center
W. Columbia, SC
-----Original Message-----
From: Jesus Ellin [mailto:JEllin <@t> yumaregional.org]
Sent: Friday, March 11, 2005 7:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC with decal
I am wondering what people are using for a decal solution that does not
interfere with IHC staining. ?? All the Help would greatly be
appreciated.
Jesus Ellin
Yuma Regional Medical Center
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 16
Date: Fri, 11 Mar 2005 09:57:37 -0500
From: "Lopez Manzano, Elisenda" <emanzano <@t> eoh.pitt.edu>
Subject: [Histonet] Question about markers for Graw Factor Antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<30B8F0F69E08A9428D634ED8866334DC1F6222 <@t> eohserver01.eoh.pitt.edu>
Content-Type: text/plain; charset="utf-8"
Hi histonetters,
I am currently working with paraffin wax embedded mouse embryos and I am
looking for markers for Graw Factor Antibody acn. I've found some
interesting ones in my bibliography, however I couldn't find these companies
on the web:
- Oncogene
- Signal transduction
Does anybody know any official website or can anybody help me finding the
price of these products?
From: Oncogene
- TGF Reference - GF 10
- TGF-R Reference - ms129091
From: Signal Transduction
- EGF-R Reference 2232
- TGF-R1 Reference 3712
Thanks!
Elisenda Lopez-Manzano
------------------------------
Message: 17
Date: Fri, 11 Mar 2005 09:24:13 -0600
From: "Lett, Jaclynn" <LettJ <@t> ent.wustl.edu>
Subject: [Histonet] Reichert Histostat manual
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<8153997545DC1A4DB92AE75F5DC34500CA0C9F <@t> EXCHANGE.wusm-pcf.wustl.edu>
Content-Type: text/plain; charset="us-ascii"
Hello,
We recently inherited a Reichert Histostat 855 made by Cambridge (the
manufacturer's label also says "975CJ Cryostat Microtome"). Its operation
manual could not be found. Does anyone have a manual and would you be
willing to send us a copy? It would be greatly appreciated.
Also, does anyone know of a disposable knife holder suitable for this model?
The steel knife holder in this machine clamps the knife on its left side.
I hope someone can assist us.
Thank you so much,
Jaclynn Lett
Senior Research Technician
Electron Microscopy Core Facility
Department of Otolaryngology
Washington University School of Medicine
660 S. Euclid Ave., Campus Box 8115
St. Louis, MO 63110
Voice: 747-7257
Fax: 747-7230
Email: lettj <@t> ent.wustl.edu
<br/>The materials in this message are private and may contain Protected
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Message: 18
Date: Fri, 11 Mar 2005 08:17:07 -0800
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: Re: [Histonet] Placenta anyone?
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <BE570483.816F%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"
When I was working at a large community hospital in the South, all placentas
were examined. For placentas of routine births an "embed & hold" was done.
Briefly, this consisted of doing a gross exam, cutting in 2-3 blocks,
processing these, & embedding the blocks. These blocks were not cut unless a
physician called & requested it. For non-routine births, the placentas were
grossed, cut, processed, sectioned & stained. We rarely froze any tissue.
Usually the placenta would be examined in a group once or twice a week. They
were held in formalin in a fridge until examined. This type of placenta
service sure seemed to make the ob/gyn types very happy.
Patti Loykasek
PhenoPath Laboratories
> Jennifer McDonald asks about placenta.
> We routinely exam every placenta from every baby born at out
> Children's & Woman's hospital. Normal placenta receive "gross only"
> treatment of weights, dimensions and description. Abnormal history or
> findings trigger a "gross in" of four blocks containing proximal,
> medial and distal cord, membranes, full thickness and sample of any
> pathological areas found. Abnormal history includes history of
> premature birth, abruption, history of more than one spontaneous
> abortion, overweight baby, etc, etc. I think examining placenta is now
> pretty much the standard of care. OBGYN people like documentation of
> placenta from problem births in case a case goes to court
>
> Mike Titford
> USA Pathology
> Mobile AL USA.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Fri, 11 Mar 2005 10:18:17 -0600
From: Don.Birgerson <@t> leica-microsystems.com
Subject: Re: [Histonet] Reichert Histostat manual
To: "Lett, Jaclynn" <LettJ <@t> ent.wustl.edu>
Cc: histonet-bounces <@t> lists.utsouthwestern.edu,
histonet <@t> pathology.swmed.edu
Message-ID:
<OF37BF044F.AF9256E4-ON86256FC1.00595781-86256FC1.00598EB0 <@t> leica-microsystem
s.com>
Content-Type: text/plain; charset=US-ASCII
Hi Jaclynn,
I will E mail a PDF copy of the operating instructions under separate
cover. If you have further questions, please phone me. Best regards, Don
Don Birgerson
Leica Microsystems
Technical Assistance Center Don.Birgerson <@t> Leica-Microsystems.Com
1-800-248-0123 ext 5918
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Message: 20
Date: Fri, 11 Mar 2005 11:23:17 -0500
From: Dana Settembre <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] Tau antibody
To: histonet <@t> lists.utsouthwestern.edu, jhofecker <@t> yahoo.com
Message-ID: <s2317fc5.082 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII
Hello Jennifer,
I use Tau from Dako with their detection kit LSAB2. But I have used other
kits in the past that work too. Vectors ABC kit and I am sure many others
work as well. We use it with an Alzheimer's brain control with no
pretreatment. I use it at a 1:800 dilution incubated for 15 minutes. Good
Luck,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
USA
>>> Jennifer Hofecker <jhofecker <@t> yahoo.com> 3/10/2005 3:14:09 PM >>>
Hi everyone,
Are any of you currently using Tau from Dako? What
detection system are you using? I Would appreciate all positive/negative
input regarding this antibody.
Also, has another supplier's Tau proven better in your experience?
Thanks in advance,
Jennifer Hofecker
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------------------------------
Message: 21
Date: Fri, 11 Mar 2005 16:22:08 -0000
From: ncragg <n.cragg <@t> epistem.co.uk>
Subject: [Histonet] F4/80 on human tumour xenografts in mouse
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <01C52656.79AB2770.n.cragg <@t> epistem.co.uk>
Content-Type: text/plain; charset="us-ascii"
Hello,
Has anyone tried murine F4/80 IHC staining on human tumour xenografts grown
in nude mice to look for infiltrating macrophages? We are using the Caltag
rat monoclonal, BM8, although we've got it working nicely on normal mouse
spleen, the staining pattern is unusual in the tumour xenografts, staining
is seen around the human tumour cells (almost like tumour matrix) rather
than infiltrating mouse cells. Has anyone seen this pattern before or can
suggest what is happening here?
Thank you in advance,
Nicola
Nicola Cragg BSc
Epistem Ltd
Manchester, UK
------------------------------
Message: 22
Date: Fri, 11 Mar 2005 09:36:34 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] IHC with decal
To: "'Carole Fields'" <cgfields <@t> lexhealth.org>, "'Jesus Ellin'"
<JEllin <@t> yumaregional.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <200503111636.j2BGaVdf026361 <@t> chip.viawest.net>
Content-Type: text/plain; charset="us-ascii"
I use Immunocal from Decal Chemicals or make my own 5% Formic Acid. Patsy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carole
Fields
Sent: Friday, March 11, 2005 7:18 AM
To: 'Jesus Ellin'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC with decal
We use Rapid-Cal Immuno from BBC. It is working fine.
Carole Fields, HT,ASCP
Pathology Supervisor
Lexington Medical Center
W. Columbia, SC
-----Original Message-----
From: Jesus Ellin [mailto:JEllin <@t> yumaregional.org]
Sent: Friday, March 11, 2005 7:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC with decal
I am wondering what people are using for a decal solution that does not
interfere with IHC staining. ?? All the Help would greatly be
appreciated.
Jesus Ellin
Yuma Regional Medical Center
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------------------------------
Message: 23
Date: Fri, 11 Mar 2005 11:38:55 -0600
From: Robin Olmscheid <rolmsche <@t> unlnotes.unl.edu>
Subject: [Histonet] workshops
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4.1.20050311113302.00b01578 <@t> unlnotes01.unl.edu>
Content-Type: text/plain; charset="us-ascii"
I would like to know if there are any special stains workshops to attend. I
know several years ago there was a week long workshop for learning and doing
special stains. This week long workshop took place some where in the south,
maybe Georgia. If anyone has any information concerning this please let me
know. Thanks.
------------------------------
Message: 24
Date: Fri, 11 Mar 2005 09:51:12 -0800
From: "Kathleen Boozer" <BoozerKA <@t> pa1.ah.org>
Subject: [Histonet] Prion Lab
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s2316a1d.059 <@t> mail1.ah.org>
Content-Type: text/plain; charset=US-ASCII
Is there a prion referral lab in the NW (Oregon)?
------------------------------
Message: 25
Date: Fri, 11 Mar 2005 09:59:17 -0800 (PST)
From: Dawn Cowie <dlcowie <@t> prodigy.net>
Subject: [Histonet] exhausting tissue blocks
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050311175917.2794.qmail <@t> web81003.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
hello histonetters,
I was wondering what thoughts any of you have on the subject of cutting away
and exhausting tissue blocks when doing recuts. We often get requests from
our pathologists to cut deepers and exhaust the block ie: exhaust in 3
slides of deepers. Does anyone know of any reg's that cover this kind of
thing? It really goes against the grain to cut away all the tissue. Plus, we
are supposed to keep the blocks for 10 years. It defeats the purpose of
doing so. Any response would be appreciated. Thanks, Dawn Cowie, HT
------------------------------
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End of Histonet Digest, Vol 16, Issue 21
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