[Histonet] MSH2 rabbit polyclonal antibody from Oncogene

Sawrenko, Christina csawrenk <@t> bccancer.bc.ca
Fri Mar 11 12:53:40 CST 2005

Good morning all!
Does anyone have experience with this antibody?  We are experiencing a
cytoplasmic "blush" in addition to very nice nuclear staining in a tumor
with a MLH1 mutation.  In order to achieve nice nuclear staining, we are
using DAKO high pH 9.9 for heat mediated antigen retrieval.  We use
EnVision+ as the detection system.   This blush may be OK so are asking
for other's experience. 
Thanks for any help you can give.
Chris Sawrenko
BC Cancer Agency
Vancouver, BC Canada

More information about the Histonet mailing list