[Histonet] goldners trichrome

[Barbara Bublava]

Hi Liz

I do Goldner routinely and there is an improvement in colour after
postfixation with bouin´s.
Muscle for example tends to bee greenish without bouin. After posttfixation
it turns out red. Every trichrome (except van Gieson) will profit from
postfixation with Bouin´s. I postfix at room temperature for at least two
hours but most often over night (i have no time pressure)

Best would be to fix in bouin only (did try that with fozens) but in routine
it is not always possible and it is said that ihc will not work after bouin
and dna is rouined ...

sorry for bad english and best wishes
Barbara

----- Original Message ----- 
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
To: "'histonet'" <histonet <@t> pathology.swmed.edu>
Sent: Tuesday, June 07, 2005 9:41 PM
Subject: [Histonet] goldners trichrome


> Hello Everyone
>
> I have to perform a goldners trichrome.  The two protocols that I have
> do not use bouins as a mordant.  One does state that the trichrome stain
> benefits from picric acid fixation but also states that 10% NBF is fine.
> Is this correct or should I mordant in bouins prior to staining.  I
> figured I would ask the histonet first before I run the stain with and
> without using bouins as a mordant.  Any advice would be helpful.
>
> Thanks in advance.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> P.O. Box 18592
> Boulder, Colorado 80308
> Office: (303) 735-5001
> Fax: (303) 735-3540
> liz <@t> premierlab.com
> www.premierlab.com
>
> Ship to Address:
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> University of Colorado
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>
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