[Histonet] CD 31

[renafail <@t> bellsouth.net]

Hi Kim,
   We use CD31 at 1:50, pretreatment HIER using Citrate buffer
Rena Fail

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim
Allred
Sent: Friday, July 22, 2005 3:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD 31

I am having a problem getting my CD31 to work.  The protocol was for an
overnight incubation and pepsin pretreatment.  I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab.  Does anyone have any ideas?  This seemed
to
work well for a while but once again, I'm having problems with it.  Any
input would be appreciated.  Thanks in advance!  

 

 

 

Kim Allred 

Dermatopathology Associates

Jackson, MS  

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