[Histonet] CD 31
Lori Richey
lrichey <@t> u.washington.edu
Fri Jul 22 17:48:27 CDT 2005
We use DAKO CD31 1:25 , 18 minutes microwave, in citrate buffer.
Kim Allred wrote:
>I am having a problem getting my CD31 to work. The protocol was for an
>overnight incubation and pepsin pretreatment. I couldn't get it to work
>this way so I altered it a bit by using trilogy for AR and a two hour
>incubation for the primary ab. Does anyone have any ideas? This seemed to
>work well for a while but once again, I'm having problems with it. Any
>input would be appreciated. Thanks in advance!
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>Kim Allred
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>Dermatopathology Associates
>
>Jackson, MS
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