[Histonet] CD 31

[Kim Allred]

I am having a problem getting my CD31 to work.  The protocol was for an
overnight incubation and pepsin pretreatment.  I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab.  Does anyone have any ideas?  This seemed to
work well for a while but once again, I'm having problems with it.  Any
input would be appreciated.  Thanks in advance!  

 

 

 

Kim Allred 

Dermatopathology Associates

Jackson, MS