Complete Endog peroxidase blocking Re: [Histonet] Endogenous peroxidase blocking

[Gayle Callis]

Pablo,

I will be sending via private email - a method that may help.  It is the 
glucose oxidase blocking method, and used nicely for frozen sections but 
also paraffin sections.

At 09:11 AM 7/22/2005, you wrote:
>Dear Histonetters,
>
>I ask for your help. I am doing an immunohistochemical procedure in really
>bloddy specimens. The endogenous peroxidase blocking solution is 3% v/v
>hydrogen peroxyde in distilled water for 15 minutes.
>
>I am afraid that this blocking is not enough. How can I be sure that I have
>totally removed the endogenous activity? Are there stronger treatments than
>mine?
>
>Should the endogenous peroxidase staining appear only in red cells and
>granulocites or could it also produce a background staining in the whole
>tissue?
>
>Best regards
>
>Pablo Sanchez
>
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)