Complete Endog peroxidase blocking Re: [Histonet] Endogenousperoxidase blocking

[pruegg <@t> ihctech.net]

I had a particularly difficult time quenching endogenous peroxidase in
sputum cytopreps full of granulocytes and it was suggested that I try the
new DAKO Dual Block for HRP and Alk.Phos. and it worked a charm, don't know
what's in it (proprietary you know), but perhaps it is similar to Gayles
reagents for glucose oxidase blocking.
Patsy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Friday, July 22, 2005 10:18 AM
To: Pablo Sánchez Quinteiro; Histonet <@t> lists.utsouthwestern.edu
Subject: Complete Endog peroxidase blocking Re: [Histonet]
Endogenousperoxidase blocking

Pablo,

I will be sending via private email - a method that may help.  It is the 
glucose oxidase blocking method, and used nicely for frozen sections but 
also paraffin sections.

At 09:11 AM 7/22/2005, you wrote:
>Dear Histonetters,
>
>I ask for your help. I am doing an immunohistochemical procedure in really
>bloddy specimens. The endogenous peroxidase blocking solution is 3% v/v
>hydrogen peroxyde in distilled water for 15 minutes.
>
>I am afraid that this blocking is not enough. How can I be sure that I have
>totally removed the endogenous activity? Are there stronger treatments than
>mine?
>
>Should the endogenous peroxidase staining appear only in red cells and
>granulocites or could it also produce a background staining in the whole
>tissue?
>
>Best regards
>
>Pablo Sanchez
>
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet