Complete Endog peroxidase blocking Re: [Histonet] Endogenousperoxidase blocking

pruegg <@t> pruegg <@t>
Sun Jul 24 15:17:39 CDT 2005

I had a particularly difficult time quenching endogenous peroxidase in
sputum cytopreps full of granulocytes and it was suggested that I try the
new DAKO Dual Block for HRP and Alk.Phos. and it worked a charm, don't know
what's in it (proprietary you know), but perhaps it is similar to Gayles
reagents for glucose oxidase blocking.

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Gayle Callis
Sent: Friday, July 22, 2005 10:18 AM
To: Pablo Sánchez Quinteiro; Histonet <@t>
Subject: Complete Endog peroxidase blocking Re: [Histonet]
Endogenousperoxidase blocking


I will be sending via private email - a method that may help.  It is the 
glucose oxidase blocking method, and used nicely for frozen sections but 
also paraffin sections.

At 09:11 AM 7/22/2005, you wrote:
>Dear Histonetters,
>I ask for your help. I am doing an immunohistochemical procedure in really
>bloddy specimens. The endogenous peroxidase blocking solution is 3% v/v
>hydrogen peroxyde in distilled water for 15 minutes.
>I am afraid that this blocking is not enough. How can I be sure that I have
>totally removed the endogenous activity? Are there stronger treatments than
>Should the endogenous peroxidase staining appear only in red cells and
>granulocites or could it also produce a background staining in the whole
>Best regards
>Pablo Sanchez
>Histonet mailing list
>Histonet <@t>

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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