[Histonet] Ventana ES for sale
Featherstone, Annette
AFeatherstone <@t> KaleidaHealth.Org
Fri Jul 22 10:01:02 CDT 2005
Is anyone out there interested in purchasing one or two working Ventana ES
stainers?
Annette Featherstone HT/MLT
Supervisor Anatomic Pathology
Kaleida Health, Buffalo General Hospital
100 High Street
Buffalo NY 14203
716-859-2625
FAX: 716-859-1853
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
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Sent: Friday, July 22, 2005 10:37
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Subject: Histonet Digest, Vol 20, Issue 29
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Today's Topics:
1. tissue falling off slides (Zhenming Yu)
2. RE: Chlamydia antibody and Aspergillus IHC (Sverlow, Karen)
3. Reagent Search: PDTS (Owen, Michael P)
4. FDC-M1 antibody (Gayle Callis)
5. CD25 on murine tissue woes (Gayle Callis)
6. Re: Reagent Search: PDTS (Gayle Callis)
7. RE: Reagent Search: PDTS (Monfils, Paul)
8. type of mouse for spleen control Re: CD25 on murine tissue
woes (Gayle Callis)
9. RE: Gram staining (Lewis, Sarah)
10. MGMT (Till, Renee)
11. Re: RE: Chlamydia antibody and Aspergillus IHC (Jan Shivers)
12. RE: CD25 on murine tissue woes (Mathew DeGutes)
13. BIOGENEX (Patsy Ruegg)
14. Murine Blood Smear / Smears IHC (GT Hebert)
15. Re: postmortem tissue collection (Y. Wang)
16. Re: Workloads vs Hiso staffing (Katia Cristina Catunda)
17. RE: Murine Blood Smear / Smears IHC (Favara, Cynthia (NIH/NIAID))
18. Black paraffin and cassettes (Steven P Postl)
19. anyone know who sells these bottles? (curt tague)
20. RE: Black paraffin and cassettes (Kristen Broomall)
21. muscle histochemistry- (bcgirish)
22. RE: Black paraffin and cassettes (Monfils, Paul)
23. RE: Black paraffin and cassettes (Pamela Marcum)
24. RE: Black paraffin and cassettes (Weems, Joyce)
25. AMACR (Edwards, R.E.)
26. glycerol (Steven Coakley)
27. RE: Black paraffin and cassettes (Favara, Cynthia (NIH/NIAID))
28. Black paraffin and cassettes (Steven P Postl)
29. RE: Black paraffin and cassettes (Rogerson Kemlo (ELHT) Pathology)
----------------------------------------------------------------------
Message: 1
Date: Thu, 21 Jul 2005 13:02:49 -0400
From: Zhenming Yu <zyu <@t> mail.med.upenn.edu>
Subject: [Histonet] tissue falling off slides
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1121965369.42dfd539b88cb <@t> webmail.pobox.upenn.edu>
Content-Type: text/plain; charset=ISO-8859-1
Thanks to so many of you for the very helpful responses to my last question
regarding chatter in fly head paraffin section. It turned out that we
probably
had over-processed the fly heads in alcohol and xylene. We are modifying our
protocols and keeping our fingers crossed that the problem will be solved
soon.
Also I got another question that I hope you guys can help with---we
sometimes
ran across the problem of patches of tissue coming off slides. Could this
also
be attributed to over exposure of the heads to alcohol and xylene during
processing? We have been using Superfrost Plus slides from Fisher---is any
further slide treatment necessary? We usually lay ribbons (5 or 7 micron
thick) of paraffin sections on the water surface in a 38°C water bath for
less
than one minute (too short?) before loading to a slide. Then the slide was
kept
in the vertical position for about 30 minutes to dry and incubated in a 37
incubator overnight for further drying.
Any thoughts would be gratefully received. Thanks.
****************************
Zhenming Yu
Univ. of Penn
Philadelphia, PA 19104
****************************
------------------------------
Message: 2
Date: Thu, 21 Jul 2005 10:20:46 -0700
From: "Sverlow, Karen" <ksverlow <@t> cahfs.ucdavis.edu>
Subject: [Histonet] RE: Chlamydia antibody and Aspergillus IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E8E4D8E33ADC6243852738D6580DF0EB838BDA <@t> predator.cahfs.ucdavis.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Everyone,
Has anyone found a replacement antibody for the Virostat Chlamydia
monoclonal product #1631 (no longer available)? Specifically, we are
looking for a monoclonal that will detect Chlamydia in FFPE mammalian
and avian tissues.
On another note, I would like to talk with those of you doing
Aspergillus IHC. Please email me.
Thank you,
Karen Sverlow
Staff Research Associate
California Animal Health and Food Safety Laboratory
University of California, Davis
ksverlow <@t> cahfs.ucdavis.edu
------------------------------
Message: 3
Date: Thu, 21 Jul 2005 10:23:58 -0700
From: "Owen, Michael P" <MICHAEL.OWEN <@t> fda.gov>
Subject: [Histonet] Reagent Search: PDTS
To: 'Histonet' <histonet <@t> pathology.swmed.edu>
Message-ID:
<DC59440775B1B84F9C81553A090398CB02A90938 <@t> orspabothell2.fda.gov>
Content-Type: text/plain
Dear Histonet Members,
A colleague of mine is looking for a reagent called "Phenolphthalein
Disulfate, Tripotassium Salt" for a staining project. The Sigma-Aldrich
product number is P0251.
Sigma-Aldrich has stated the chemical is no longer available. Does anyone on
this list know of an alternative source?
Thanks in advance.
Michael P. Owen, Regulatory Microbiologist
U.S. FDA Pacific Regional Lab Northwest
22201 23rd Drive SE Bothell, WA 98021-4421
Phone: 425-483-4865 E-Mail: michael.owen <@t> fda.gov
------------------------------
Message: 4
Date: Thu, 21 Jul 2005 11:27:05 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] FDC-M1 antibody
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050721112507.01b18438 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Cynthia,
Yes, we stain for this using IFA, but it also works IHC. We use frozens
sections only, and it was an easy protocol. The antibody came from BD
Pharmingen.
You wrote:
Does anyone have any experience with FDC[follicular dendritic cell] -M1
antibody clone 4C11 in mouse tissue?I do have some references that I am
tracking down, but am having difficulty with the source
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 5
Date: Thu, 21 Jul 2005 11:33:08 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] CD25 on murine tissue woes
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050721112807.01b13c90 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Matthew,
You wrote:
I am trying to get CD25 staining to work in order to identify T reg cells
in mouse tissue sections. So far I really haven't had much luck. I have
both a 7D4 biotinylated anti-CD25 from Pharmingen and a PC61
Fitc-conjugated anti-CD25 from eBioscience and I am using Spleen as a
positive control.
I am using fresh frozen sections that have not been fixed at all. I have
tried various fixations with Acetone and PFA. I would think PFA might
block staining as many people have had trouble getting staining on parafin
sections. I have played with many different antibody concentrations and we
have secondaries and amplification protocols that have worked for other
biotinylated and fitc-conjugated antibodies quite well using strep-hrp and
anti-fitc-hrp along with a TSA cy3 or fluorescein kit. Does anyone have a
protocol or some tips on making these antibodies stain for
immunofluorescence? I know this is a common stain, but I simply have had
no luck with it. Any help is appreciated thanks.
Could you provide more details on HOW you are doing the staining, both as
IHC and or immunofluorescence. If you are trying to do the primary
antibody directly conjugated to FITC on a tissue section, you may not get
positive results - this is not uncommon with some of this type conjugate as
a direct IFA stain. I can't make it work with CD4-FITC or CD8-FITC.
As for biotinylated antibodies (primaries) one can do either IHC and IFA -
we do this on many other rat antimouse primaries, CD markers - so provide
concentrations, times, etc, etc - all those good things.
Thanks
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 6
Date: Thu, 21 Jul 2005 11:40:16 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Re: Reagent Search: PDTS
To: "Owen, Michael P" <MICHAEL.OWEN <@t> fda.gov>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050721113905.01b6cf68 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Try some other chemical companies - ACROS vis Fisher, and ICN are
possibilities.
At 11:23 AM 7/21/2005, you wrote:
>Dear Histonet Members,
>
>A colleague of mine is looking for a reagent called "Phenolphthalein
>Disulfate, Tripotassium Salt" for a staining project. The Sigma-Aldrich
>product number is P0251.
>
>Sigma-Aldrich has stated the chemical is no longer available. Does anyone
on
>this list know of an alternative source?
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 7
Date: Thu, 21 Jul 2005 13:51:08 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Reagent Search: PDTS
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE0171757F <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="ISO-8859-1"
Lab Depot carries this compound. www.labdepotinc.com
Also, you can search for suppliers of chemicals at www.chemexper.com
Paul M.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Owen,
> Michael P
> Sent: Thursday, July 21, 2005 10:23 AM
> To: 'Histonet'
> Subject: [Histonet] Reagent Search: PDTS
>
>
>
> Dear Histonet Members,
>
> A colleague of mine is looking for a reagent called "Phenolphthalein
> Disulfate, Tripotassium Salt" for a staining project. The Sigma-Aldrich
> product number is P0251.
>
> Sigma-Aldrich has stated the chemical is no longer available. Does anyone
> on
> this list know of an alternative source?
>
> Thanks in advance.
>
>
> Michael P. Owen, Regulatory Microbiologist
> U.S. FDA Pacific Regional Lab Northwest
> 22201 23rd Drive SE Bothell, WA 98021-4421
> Phone: 425-483-4865 E-Mail: michael.owen <@t> fda.gov
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 8
Date: Thu, 21 Jul 2005 11:54:25 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] type of mouse for spleen control Re: CD25 on
murine tissue woes
To: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050721115143.01b4be20 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Mouse was C57 black.
At 11:44 AM 7/21/2005, you wrote:
>Just curious, Gayle - what type of mouse is your spleen from?
>
>Jacqueline M. O'Connor HT(ASCP) QIHC
>Assistant Scientist
>Discovery Cancer R4N2 AP3
>847-938-4919
>Jackie.O'Connor <@t> abbott.com
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 9
Date: Thu, 21 Jul 2005 13:55:54 -0400
From: "Lewis, Sarah" <LewisS <@t> ccri.net>
Subject: RE: [Histonet] Gram staining
To: <jcarpenter764 <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<714B9F12B4E18C4C843B66E8E190F2AD44755C <@t> res2k3ms01.CRII.ORG>
Content-Type: text/plain; charset="iso-8859-1"
rumor has it that *slim Jim* beef jerky works for both gram positive and
gram negative.>>>??
-----Original Message-----
From: jcarpenter764 <@t> aol.com [mailto:jcarpenter764 <@t> aol.com]
Sent: Thursday, July 21, 2005 10:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gram staining
Can anyone give me some tips on how to get Gram stain controls or how to
substitute for the control. Thanks Jennell
------------------------------
Message: 10
Date: Thu, 21 Jul 2005 14:19:32 -0500
From: "Till, Renee" <TillRenee <@t> uams.edu>
Subject: [Histonet] MGMT
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<EB352EB477F2064285B7B2192E37CC28096E746B <@t> exchpeds.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii
Hello. Does anyone know of a good MGMT antibody for rat tissues? I have
found several that say weakly reactive with rat tissue, and many that
don't specify.
Renee' Till, HT
Arkansas Childrens Nutrition Center
1120 Marshall
Little Rock, AR
72202
(501) 364-2774
------------------------------
Message: 11
Date: Thu, 21 Jul 2005 14:33:34 -0500
From: "Jan Shivers" <shive003 <@t> umn.edu>
Subject: Re: [Histonet] RE: Chlamydia antibody and Aspergillus IHC
To: "Sverlow, Karen" <ksverlow <@t> cahfs.ucdavis.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00c801c58e2b$15c5ce30$41065486 <@t> auxs.umn.edu>
Content-Type: text/plain; charset="iso-8859-1"
I don't use a monoclonal for Chlamydia, but I do use a rabbit polyclonal
from Biodesign (B65256R). It's made against Chlamydia trachomatis (EB's all
antigens), but cross-reacts with Chlamydia psittacii and pneumoniae. It
requires no tissue pretreatment.
Jan Shivers
Univ. of Minnesota Vet Diag Lab
----- Original Message -----
From: "Sverlow, Karen" <ksverlow <@t> cahfs.ucdavis.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, July 21, 2005 12:20 PM
Subject: [Histonet] RE: Chlamydia antibody and Aspergillus IHC
Hi Everyone,
Has anyone found a replacement antibody for the Virostat Chlamydia
monoclonal product #1631 (no longer available)? Specifically, we are
looking for a monoclonal that will detect Chlamydia in FFPE mammalian
and avian tissues.
On another note, I would like to talk with those of you doing
Aspergillus IHC. Please email me.
Thank you,
Karen Sverlow
Staff Research Associate
California Animal Health and Food Safety Laboratory
University of California, Davis
ksverlow <@t> cahfs.ucdavis.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Thu, 21 Jul 2005 15:00:54 -0500
From: Mathew DeGutes <m-degutes <@t> northwestern.edu>
Subject: [Histonet] RE: CD25 on murine tissue woes
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <200507212001.j6LK14Mv008154 <@t> casbah.it.northwestern.edu>
Content-Type: text/plain
If you want all the nitty gritty details. General protocol is this
1. Cut sections. Let dry and store at -80c until use.
2. Let sections return to room temperature and then fix. (Have tried Acetone
10 min -20c, 2%PFA 5
min room temp)
3. Wash 3x5 PBS
4. Wash 5 min in 3% H2O2 (have tried without this step)
5. Wash 3x5 PBS
6. Encircle and Avidin Block 10 min. (avidin biotin blocks are dependent on
whether a biotinylated
antibody is used).
7. Wash off Avidin in PBS
8. Biotin block 10 min
10. Aspirate Biotin block and add Block (have tried various serum at 10% and
TNB, either with
CD16/32 added at 1:50) for 30 min minimum.
11. Aspirate block and add Primary ab (have tried both 7D4 biotinylated
anti-CD25 from Pharmingen
and a PC61 Fitc-conjugated anti-CD25 from eBioscience at concentrations from
1:25 to 1:500) diluted
in TNB or a 2% serum + .2% Triton in PBS buffer for 1 hour at room temp
(have also tried overnight
at 4c, but generally do the 1hour room temp).
12. Wash 3x5 PBS
13. Add 2ndary ab (Either Streptavidin-HRP or Anti-Fitc HRP) 1:100 in
staining buffer from step 11
and inc for 30 min.
14. Wash 3x5 PBS
15. Use TSA Cy3 or Fluorescein kit. Dilute 1:100 in diluent and develop 5
minutes.
16. Wash 3x5 PBS
17. Mount with Vectashield with DAPI and coverslip
Now we have used these 2ndaries and TSA kits extensively and have found them
to work very well on
any number of antigens. As I said before I haven't attempted to make it
work with a direct. I
come back and amplify with anti-fitc-hrp and the TSA kit. Also we are
generally working in SJL
mouse strains. I would really like to get this to work to go alongside
FoxP3 staining for regs. I
realize that regs are supposedly lower in SJL strains, but I have attempted
this on Naive BalbC
spleens as well and also have attempted on spleens from mice that were given
injections of CD25+
CD4+ Tcells and so should have a far greater number of cells that are CD25+
(whether these cells
will actually show up in anything other than flow I am not sure).
You Wrote:
Could you provide more details on HOW you are doing the staining, both as
IHC and or immunofluorescence. If you are trying to do the primary
antibody directly conjugated to FITC on a tissue section, you may not get
positive results - this is not uncommon with some of this type conjugate as
a direct IFA stain. I can't make it work with CD4-FITC or CD8-FITC.
As for biotinylated antibodies (primaries) one can do either IHC and IFA -
we do this on many other rat antimouse primaries, CD markers - so provide
concentrations, times, etc, etc - all those good things.
------------------------------
Message: 13
Date: Thu, 21 Jul 2005 14:23:42 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] BIOGENEX
To: "'Histonet'" <histonet <@t> pathology.swmed.edu>
Message-ID: <200507212023.j6LKNepf006377 <@t> chip.viawest.net>
Content-Type: text/plain; charset="US-ASCII"
Can someone from BioGenex please contact me about a MW of theirs I am
demoing.
Patsy Ruegg
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/>
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
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you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
------------------------------
Message: 14
Date: Thu, 21 Jul 2005 15:30:22 -0700 (PDT)
From: GT Hebert <emerald_lake77 <@t> yahoo.com>
Subject: [Histonet] Murine Blood Smear / Smears IHC
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050721223022.30674.qmail <@t> web31710.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello everyone,
I've searched but my answers so far have come up too vague.
I need to know if it is possible to do IHC on mouse blood smears???? If so,
how would one go about preparing and fixing the sample? Is there anything
I should watch out for?
I just want to run normal IHC (probably to mark certain white blood cells
(for example, monocytes to start).
Any assistance is valued and greatly appreciated. Thanks!
Gustave Hebert
Scientist II
Wyeth Research
Cambridge MA
__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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------------------------------
Message: 15
Date: Thu, 21 Jul 2005 15:47:19 -0700 (PDT)
From: "Y. Wang" <ynwang <@t> u.washington.edu>
Subject: Re: [Histonet] postmortem tissue collection
To: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu, "Rogerson Kemlo \(ELHT\)
Pathology" <Kemlo.Rogerson <@t> elht.nhs.uk>
Message-ID:
<Pine.A41.4.61b.0507211535420.326972 <@t> homer03.u.washington.edu>
Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
Thank you all for your thoughts on this question. I haven't found anything
more enlightening on the web searches I've done so far. However, I will
continue searching and will let you know if and when I find some thing
exciting (aside from the strange but interesting nuggets that one always
happens across in these searches on tissue death after death).
Thanks again
Yak-Nam
> Many years ago (I hate beginning my responses like that but it is true)
> several colleagues were able to get viable human retinal pigment
epithelial
> cells more than 48 hours after death. Also, I know of viable olfactory
> (sensory) epithelial cells being harvested 24 hours after death. Of
course,
> ambient temperature is a likely variable and not all cells die at the same
> time. I would think that the adipose tissue in the hypodermis would have a
> relatively slow metabolic rate and might be viable for 24 hours? You have
> little to lose by trying.
>
> Geoff
>
> Rogerson Kemlo (ELHT) Pathology wrote:
>
>> Interestingly I was reading about tissue death after death (so as to
>> speak) on a Web Site dealing with Death (I'm strange like that); I was
>> interested in rigor. I hadn't realised that some tissue deep within the
>> cadaver can survive for many hours after death. I suppose it's those
>> tissues that require little or no oxygen to survive; brain dies very
>> rapidly. If I could remember the Site then I'd tell you but a search in
>> Google under rigor may help. Wonder which bit of you dies last? Same in
>> men and women?
>>
>> -----Original Message-----
>> From: Y. Wang [mailto:ynwang <@t> u.washington.edu] Sent: 20 July 2005 00:20
>> To: Histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] postmortem tissue collection
>>
>> Dear histonetters,
>>
>> I have a question regarding collection of human tissue. A colleague
>> would like to collect human tissue (skin and underlying adipose tissue)
for
>> mechanical testing, histological analysis (cellular and structural
>> evaluation), IHC and protein analysis (extracellular matrix structure
>> and concentrations). They asked what would be a fair cut off time for
>> tissue
>>
>> collection so that the effects of decay would not be a factor. Currently
>>
>> they have given the tissue bank a time of 12 hours postmortem (I'm not
very
>> sure how the body is stored during this time or how this is calculated).
>>
>> I've read that human decomposition starts approx. 4 min after death and
>> autolysis is quicker in tissues with high enzyme and water content.
>> However, in terms of the skin and underlying adipose tissue I didn't
>> know if there was an accepted 'cut off time' postmortem after which
tissue
>> is considered far from representative of 'live' tissue and not worth
>> analysis.
>>
>> Can anyone give some insight? Any information or references would be
>> greatly appreciated.
>>
>> Thank you
>> Yak-Nam
>>
>> Senior Fellow
>> Department of Bioengineering
>> University of Washington
>> Box 357962
>> Seattle, WA 98195
>>
>> Tel.: (206)-221-5873
>> Fax.: (206)-221-5874
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029 mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
------------------------------
Message: 16
Date: Thu, 21 Jul 2005 20:22:32 -0300
From: "Katia Cristina Catunda" <kccatunda <@t> terra.com.br>
Subject: Re: [Histonet] Workloads vs Hiso staffing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <015b01c58e4b$12750f00$a279fea9 <@t> privatexx>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
We do have a great interest on that question either. My supervisor sent me
an FAQ he got on CAP telling that the ideal workload would be 50 H/E slides
per histotechnician
http://www.cap.org/apps/docs/cap_today/q_and_a/qa_1102.html - answered by
Richard W. Brown, MD
In our lab we do have 12 histotechnicians with a routine that is about 470
blocks (for 1/3 of them we generate two slides, one for HE and one for H.
pilory) and 600 citological slides per day. They alternate their function
every week:
- embedding - 3 of them
- microtomy 4 of them
- staining/mounting and organizing slides - 2 of them
- specific staining -the same of embedding
- archiving blocks - the same of staining
- citology routine - 2 of them
- one is allways on vacation
- immunohistochemistry - 2 of them are moved to other room twice a week
I was wondering which is the best way to determinate their workload in order
to analyse if we have too many or too less employees. My personal opinion is
that 50 H/E slides per employee would be very hard-working and out of our
range.
Does anybody has any idea?
But all of these informations are international since we are from Brazil...
Katia
----- Original Message -----
From: "Maray Weirauch" <mweirauch <@t> crittenton.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, July 18, 2005 3:44 PM
Subject: [Histonet] Workloads vs Hiso staffing
> ** PRIVATE **
>
> Our hospital is conducting an analysis of laboratory workload, workflow
> and staffing. This will include Histology as well, and we are being
> asked for input with any national benchmarks available for surgical
> histology tasks (i.e. average number cases accessioned, blocks embedded,
> slides cut../some type of time unit) Does anyone know of some realistic
> published values or ranges? Thanks!
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 17
Date: Thu, 21 Jul 2005 20:15:57 -0400
From: "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov>
Subject: RE: [Histonet] Murine Blood Smear / Smears IHC
To: 'GT Hebert' <emerald_lake77 <@t> yahoo.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<A985B7D45D355D4EB363288E7173943709DE6B <@t> NIHCESMLBX5.nih.gov>
Content-Type: text/plain
Questions - why are you dong this? What is the ultimate goal? If you just
want a differential then do a differential. If you are looking for various
populations of cells then FACS might be more reasonable and far simpler.
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
Disclaimer:
The information in this e-mail and any of its attachments is confidential
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-----Original Message-----
From: GT Hebert [mailto:emerald_lake77 <@t> yahoo.com]
Sent: Thursday, July 21, 2005 3:30 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Murine Blood Smear / Smears IHC
Hello everyone,
I've searched but my answers so far have come up too vague.
I need to know if it is possible to do IHC on mouse blood smears???? If so,
how would one go about preparing and fixing the sample? Is there anything
I should watch out for?
I just want to run normal IHC (probably to mark certain white blood cells
(for example, monocytes to start).
Any assistance is valued and greatly appreciated. Thanks!
Gustave Hebert
Scientist II
Wyeth Research
Cambridge MA
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------------------------------
Message: 18
Date: Thu, 21 Jul 2005 19:40:26 -0500
From: "Steven P Postl" <steven.p.postl <@t> abbott.com>
Subject: [Histonet] Black paraffin and cassettes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF3985725A.E0D4DE05-ON86257046.0002FA1D <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
A researcher is wondering (now so am I [I never heard this request
before]) if any company sells black paraffin and black embedding
cassettes? No, this isn't a Gothic thing, a legitimate request. Would it
be possible to add something to paraffin to make it stay a black color and
remain strong as our commercial paraffin products? Thanks.
------------------------------
Message: 19
Date: Thu, 21 Jul 2005 18:05:31 -0700 (PDT)
From: curt tague <opiecurt <@t> yahoo.com>
Subject: [Histonet] anyone know who sells these bottles?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050722010531.85013.qmail <@t> web80101.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
there are 2 picutres under histo net images. i'd love to find a vendor for
these bottles. others are a tad too small for my labels.
thanks.
------------------------------
Message: 20
Date: Fri, 22 Jul 2005 08:24:34 -0400
From: "Kristen Broomall" <kbroomal <@t> NEMOURS.ORG>
Subject: RE: [Histonet] Black paraffin and cassettes
To: "'Steven P Postl'" <steven.p.postl <@t> abbott.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6E41111281623B4B8A9AB8F9A7EA3437824394 <@t> wlmmsx02.nemours.org>
Content-Type: text/plain; charset=iso-8859-1
I would think it would be difficult to find something to write on a black
cassette & be visible.
Kristen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Steven P
Postl
Sent: Thursday, July 21, 2005 8:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Black paraffin and cassettes
A researcher is wondering (now so am I [I never heard this request
before]) if any company sells black paraffin and black embedding
cassettes? No, this isn't a Gothic thing, a legitimate request. Would it
be possible to add something to paraffin to make it stay a black color and
remain strong as our commercial paraffin products? Thanks.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: 22 Jul 2005 13:21:05 -0000
From: "bcgirish" <bcgpath <@t> rediffmail.com>
Subject: [Histonet] muscle histochemistry-
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050722132105.13765.qmail <@t> webmail29.rediffmail.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Bernice Gut Schmidt
I am very much interested in the method you are using for muscle atpse
histochemistry(Dubowitz and Brooke).If you can send me the same it will be
very much useful to us.
My Fax ------91-40-23045438
Phone ----914023045439
REGARDS
Dr.Girish B.Chandrashekar
Dept of Preclinical Safety Evaluation
Dr Reddys Lab
Hyderabad-49
------------------------------
Message: 22
Date: Fri, 22 Jul 2005 10:10:58 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Black paraffin and cassettes
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE01717580 <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="ISO-8859-1"
I believe the dye Sudan Black B is soluble in paraffin.
Black cassettes? Nope.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Steven P Postl
> Sent: Thursday, July 21, 2005 5:40 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Black paraffin and cassettes
>
> A researcher is wondering (now so am I [I never heard this request
> before]) if any company sells black paraffin and black embedding
> cassettes? No, this isn't a Gothic thing, a legitimate request. Would it
>
> be possible to add something to paraffin to make it stay a black color and
>
> remain strong as our commercial paraffin products? Thanks.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 23
Date: Fri, 22 Jul 2005 10:16:24 -0400
From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
Subject: RE: [Histonet] Black paraffin and cassettes
To: "Monfils, Paul" <PMonfils <@t> Lifespan.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6.1.1.1.2.20050722101403.019bfb80 <@t> mail.vet.upenn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
At 10:10 AM 7/22/2005, Monfils, Paul wrote:
>I believe the dye Sudan Black B is soluble in paraffin.
>
>Black cassettes? Nope.
>
> > ----------
> > From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> > Steven P Postl
> > Sent: Thursday, July 21, 2005 5:40 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Black paraffin and cassettes
> >
> > A researcher is wondering (now so am I [I never heard this request
> > before]) if any company sells black paraffin and black embedding
> > cassettes? No, this isn't a Gothic thing, a legitimate request. Would
it
> >
> > be possible to add something to paraffin to make it stay a black color
and
> >
> > remain strong as our commercial paraffin products? Thanks.
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
It is soluble in paraffin however it will be removed by reagents used in
deparaffinization and staining. Due to the additional materials added to
raw paraffin it will be spotty. It will also leave some precipitate in the
tissue.
Best Regards,
Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr. CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348
Phone - 610-925-6278
Fax - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu
------------------------------
Message: 24
Date: Fri, 22 Jul 2005 10:16:54 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Black paraffin and cassettes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<83AACDB0810528418AA106F9AE9B7F7EA461D8 <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
Perhaps clear cassettes would work?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Monfils,
Paul
Sent: Friday, July 22, 2005 10:11 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Black paraffin and cassettes
I believe the dye Sudan Black B is soluble in paraffin.
Black cassettes? Nope.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Steven P Postl
> Sent: Thursday, July 21, 2005 5:40 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Black paraffin and cassettes
>
> A researcher is wondering (now so am I [I never heard this request
> before]) if any company sells black paraffin and black embedding
> cassettes? No, this isn't a Gothic thing, a legitimate request. Would it
>
> be possible to add something to paraffin to make it stay a black color and
>
> remain strong as our commercial paraffin products? Thanks.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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------------------------------
Message: 25
Date: Fri, 22 Jul 2005 15:17:34 +0100
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] AMACR
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DC88BEDFD1FC3F468D0376A7C75465F705C754BC <@t> Saffron.cfs.le.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Looking for an antibody to alpha-methylacyl co-enzyme A racemose(AMACR)
which works on paraffin sections of mouse tissues.
Many thanks
Richard Edwards
MRC TOXICOLOGY UNIT
LEICESTER.U.K.....
------------------------------
Message: 26
Date: Fri, 22 Jul 2005 07:22:10 -0700 (PDT)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] glycerol
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050722142210.84803.qmail <@t> web90203.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I need to have someone refresh my memory why some special stains call for
glycerol?
Steve
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------------------------------
Message: 27
Date: Fri, 22 Jul 2005 10:28:03 -0400
From: "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov>
Subject: RE: [Histonet] Black paraffin and cassettes
To: 'Steven P Postl' <steven.p.postl <@t> abbott.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<A985B7D45D355D4EB363288E7173943709DE6C <@t> NIHCESMLBX5.nih.gov>
Content-Type: text/plain
Osmium will make everything black!
c
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
Disclaimer:
The information in this e-mail and any of its attachments is confidential
and may contain sensitive information. It should not be used by anyone who
is not the original intended recipient. If you have received this e-mail in
error please inform the sender and delete it from your mailbox or any other
storage devices. National Institute of Allergy and Infectious Diseases shall
not accept liability for any statements made that are sender's own and not
expressly made on behalf of the NIAID by one of its representatives
-----Original Message-----
From: Steven P Postl [mailto:steven.p.postl <@t> abbott.com]
Sent: Thursday, July 21, 2005 5:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Black paraffin and cassettes
A researcher is wondering (now so am I [I never heard this request
before]) if any company sells black paraffin and black embedding
cassettes? No, this isn't a Gothic thing, a legitimate request. Would it
be possible to add something to paraffin to make it stay a black color and
remain strong as our commercial paraffin products? Thanks.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 28
Date: Fri, 22 Jul 2005 09:31:55 -0500
From: "Steven P Postl" <steven.p.postl <@t> abbott.com>
Subject: [Histonet] Black paraffin and cassettes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF2EC5A6C5.8C24D86F-ON86257046.004F166C <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
Let me add a few points after touching base with the researcher. There
will be no tissue processed, nor labeling needed. Just a plain paraffin
block made. This block will be used like a "cork board" but with paraffin
instead to pin a tissue flat to the surface. Odd request, but I have to
ask. Thanks again for those who have answered, and thanks to all of you
who are scratching your head, drinking your coffee and thinking about
this. TGIF
----- Forwarded by Steven P Postl/LAKE/PPRD/ABBOTT on 07/22/2005 09:24 AM
-----
Steven P Postl
07/21/2005 07:40 PM
To: histonet <@t> lists.utsouthwestern.edu
cc:
Subject: Black paraffin and cassettes
A researcher is wondering (now so am I [I never heard this request
before]) if any company sells black paraffin and black embedding
cassettes? No, this isn't a Gothic thing, a legitimate request. Would it
be possible to add something to paraffin to make it stay a black color and
remain strong as our commercial paraffin products? Thanks.
------------------------------
Message: 29
Date: Fri, 22 Jul 2005 15:34:59 +0100
From: "Rogerson Kemlo (ELHT) Pathology" <Kemlo.Rogerson <@t> elht.nhs.uk>
Subject: RE: [Histonet] Black paraffin and cassettes
To: "Monfils, Paul" <PMonfils <@t> Lifespan.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<53A22BBB01D6184EBF7A4DC18A021E9E698DF6 <@t> elht-exch1.xelht.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
That's a bit like black bed sheets; a trifle strange!
-----Original Message-----
From: Monfils, Paul [mailto:PMonfils <@t> Lifespan.org]
Sent: 22 July 2005 15:11
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Black paraffin and cassettes
I believe the dye Sudan Black B is soluble in paraffin.
Black cassettes? Nope.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Steven P Postl
> Sent: Thursday, July 21, 2005 5:40 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Black paraffin and cassettes
>
> A researcher is wondering (now so am I [I never heard this request
> before]) if any company sells black paraffin and black embedding
> cassettes? No, this isn't a Gothic thing, a legitimate request.
Would it
>
> be possible to add something to paraffin to make it stay a black color
and
>
> remain strong as our commercial paraffin products? Thanks.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 20, Issue 29
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