[Histonet] RE: Cytology Immuno's
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Wed Jul 20 04:42:59 CDT 2005
Despite previous posts on this subject, to my opinion using paraffin
sections as positive control along with cytology cytospin preps is not
such a good idea! That also accounts for paraffin cytoblocks as
positive control. I do understand that finding appropriate positive
controls here isn't easy from practical point of view.
My point is that a cyotology cytospin prep consists of cells that are
(more or less) intact with an outer membrane, whereas paraffin
sections from tissue or cytoblocks contain cut-open cells and no outer
membrane. We have observed here quite dramatic differences between
intact cells and cut-open cells when performing IHC techniques. This
has everything to do with the cellular outer membrane as barrier
for your antibodies. I think that immunostaining of intact cells,
needs intact cells as positive control. And, immunostaining of
sections, needs sections as positive control. Having said that, I also
think we have to go back to the original terminology:
"immunocytochemistry" for cells and "immunohistochemistry" for tissue
sections. Mixing up those terms long time ago was a pretty bad idea.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
NL-1105 AZ Amsterdam
phone: +31 20 5665631
fax: +31 20 6960389
Date: Tue, 19 Jul 2005 15:40:51 +0100
From: Jean Gillson <jean.gillson <@t> elht.nhs.uk>
Subject: [Histonet] Cytology Immuno's
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Can I ask you all, what do you use as controls when doing ICC on
cytospin preps or smears? Since there isn't much sample, do you use
paraffin sections as positive controls and is that adequate control
measures? We currently only use paraffin cytoblocks but considering
thin layer cytology preps and wondering what control material to use.
Thanks for your help.
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