[Histonet] Problem with Eosin Y staining

Roy Ellis roy.ellis <@t> imvs.sa.gov.au
Tue Jul 19 17:20:52 CDT 2005

Hi Michael
Alkaline mountants will bleed eosin from the section into the mounting
medium. I suggest you change your mounting medium for one that is known to
be neutral.
Roy Ellis
mailto:roy.ellis <@t> imvs.sa.gov.au

> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
> Habitzruther, Michael
> Sent: Wednesday, 20 July 2005 12:27 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: Demant, Peter
> Subject: [Histonet] Problem with Eosin Y staining
> Our laboratory is having a problem with the staining of mouse
> tumor samples using hemotoxylin and Eosin Y (H&E Stain).
> Everything was going fine until recently.  The specific problem
> we are having is after the cover-slip is put on and the acrymount
> dries, the Eosin Y stain seems to be bleeding out, leaving only
> the hemotoxylin staining.  A histologist from the institute
> suggested it may have something to do with the relative humidity
> in our lab, and therefore allowing the slides to set under a
> flow-hood may help.  Any other suggestions or protocol
> modifications would be greatly appreciated.  Thank you very much.
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