[Histonet] Problem with Eosin Y staining

[Rogerson Kemlo (ELHT) Pathology]

Eosin is soluble in both water and alcohol isn't it? Well if it bleeds
out one of these must be present mustn't it? Solution (forgive the pun)
is to eradicate both; I suspect it's alcohol.

-----Original Message-----
From: Habitzruther, Michael
[mailto:Michael.Habitzruther <@t> RoswellPark.org] 
Sent: 19 July 2005 15:57
To: histonet <@t> lists.utsouthwestern.edu
Cc: Demant, Peter
Subject: [Histonet] Problem with Eosin Y staining

Our laboratory is having a problem with the staining of mouse tumor
samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going
fine until recently.  The specific problem we are having is after the
cover-slip is put on and the acrymount dries, the Eosin Y stain seems to
be bleeding out, leaving only the hemotoxylin staining.  A histologist
from the institute suggested it may have something to do with the
relative humidity in our lab, and therefore allowing the slides to set
under a flow-hood may help.  Any other suggestions or protocol
modifications would be greatly appreciated.  Thank you very much.


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