[Histonet] Problem with Eosin Y staining

Rogerson Kemlo (ELHT) Pathology Kemlo.Rogerson <@t> elht.nhs.uk
Tue Jul 19 10:05:25 CDT 2005

Eosin is soluble in both water and alcohol isn't it? Well if it bleeds
out one of these must be present mustn't it? Solution (forgive the pun)
is to eradicate both; I suspect it's alcohol.

-----Original Message-----
From: Habitzruther, Michael
[mailto:Michael.Habitzruther <@t> RoswellPark.org] 
Sent: 19 July 2005 15:57
To: histonet <@t> lists.utsouthwestern.edu
Cc: Demant, Peter
Subject: [Histonet] Problem with Eosin Y staining

Our laboratory is having a problem with the staining of mouse tumor
samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going
fine until recently.  The specific problem we are having is after the
cover-slip is put on and the acrymount dries, the Eosin Y stain seems to
be bleeding out, leaving only the hemotoxylin staining.  A histologist
from the institute suggested it may have something to do with the
relative humidity in our lab, and therefore allowing the slides to set
under a flow-hood may help.  Any other suggestions or protocol
modifications would be greatly appreciated.  Thank you very much.

This email message may contain legally privileged and/or confidential
information.  If you are not the intended recipient(s), or the employee
or agent responsible for the delivery of this message to the intended
recipient(s), you are hereby notified that any disclosure, copying,
distribution, or use of this email message is prohibited.  If you have
received this message in error, please notify the sender immediately by
e-mail and delete this email message from your computer. Thank you.

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list