[Histonet] Problem with Eosin Y staining

[Jennifer MacDonald]

Is this a new mounting medium that you are using, or a new clearing agent? 
 We had problems with eosin bleeding when the mounting medium and the 
clearing agent were not compatible.
Jennifer MacDonald




"Habitzruther, Michael" <Michael.Habitzruther <@t> RoswellPark.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/19/2005 07:56 AM

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<histonet <@t> lists.utsouthwestern.edu>
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"Demant, Peter" <Peter.Demant <@t> RoswellPark.org>
Subject
[Histonet] Problem with Eosin Y staining






Our laboratory is having a problem with the staining of mouse tumor 
samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going 
fine until recently.  The specific problem we are having is after the 
cover-slip is put on and the acrymount dries, the Eosin Y stain seems to 
be bleeding out, leaving only the hemotoxylin staining.  A histologist 
from the institute suggested it may have something to do with the relative 
humidity in our lab, and therefore allowing the slides to set under a 
flow-hood may help.  Any other suggestions or protocol modifications would 
be greatly appreciated.  Thank you very much.


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