[Histonet] Problem with Eosin Y staining

[Joyce Cline]

We have a high humidity problem and I use H-2 Blue beads to remove water
from our clearing agent. We check and change our alcohols after our
eosin everyday. Our clearing agent will be opaque if not checked
frequently. (water in the clearing agent will cause Eosin to bleed)
*********************************************************************

Our laboratory is having a problem with the staining of mouse tumor
samples using hemotoxylin and Eosin Y (H&E Stain).  Everything was going
fine until recently.  The specific problem we are having is after the
cover-slip is put on and the acrymount dries, the Eosin Y stain seems to
be bleeding out, leaving only the hemotoxylin staining.  A histologist
from the institute suggested it may have something to do with the
relative humidity in our lab, and therefore allowing the slides to set
under a flow-hood may help.  Any other suggestions or protocol
modifications would be greatly appreciated.  Thank you very much.


This email message may contain legally privileged and/or confidential
information.  If you are not the intended recipient(s), or the employee
or agent responsible for the delivery of this message to the intended
recipient(s), you are hereby notified that any disclosure, copying,
distribution, or use of this email message is prohibited.  If you have
received this message in error, please notify the sender immediately by
e-mail and delete this email message from your computer. Thank you.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system.