[Histonet] PCR and ISH in fixed tissues

Caroline Bass cbass <@t> bidmc.harvard.edu
Mon Jul 4 12:54:08 CDT 2005

Hey guys,

My boss is submitting a grant and he does not believe that you can do 
PCR (RT) or ISH with fixed tissues.  I thought you could but I don't 
know any details.  Could someone elaborate?  Is there a particular way 
the tissue should be fixed?  In other words do I have to use FFPE 
sections or can I use 4% paraformaldehyde perfusion?

Any advice would be helpful.


Caroline Bass

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