[Histonet] PCR and ISH in fixed tissues

[Caroline Bass]

Hey guys,

My boss is submitting a grant and he does not believe that you can do 
PCR (RT) or ISH with fixed tissues.  I thought you could but I don't 
know any details.  Could someone elaborate?  Is there a particular way 
the tissue should be fixed?  In other words do I have to use FFPE 
sections or can I use 4% paraformaldehyde perfusion?

Any advice would be helpful.

Thanks,

Caroline Bass