[Histonet] PCR and ISH in fixed tissues
cbass <@t> bidmc.harvard.edu
Mon Jul 4 12:54:08 CDT 2005
My boss is submitting a grant and he does not believe that you can do
PCR (RT) or ISH with fixed tissues. I thought you could but I don't
know any details. Could someone elaborate? Is there a particular way
the tissue should be fixed? In other words do I have to use FFPE
sections or can I use 4% paraformaldehyde perfusion?
Any advice would be helpful.
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