[Histonet] formic acid decal

[clifford berger]

For more information on this product, technical papers and much more information, you can visit our website, www.decal-bone.com. 

You can also call us at 1-800-428-5856 for free samples.  

Best regards and happy 4rth

Cliff Berger
Decal Chemical Corp
The Real Decal
1-800-428-5856


----- Original Message ----- 
From: "patsy ruegg" <pruegg <@t> ihctech.net>
To: <lpwenk <@t> sbcglobal.net>; "'louise renton'" <louise.renton <@t> gmail.com>; <Histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, July 04, 2005 11:24 AM
Subject: RE: [Histonet] formic acid decal


> For I IHC I use ImmunoCal for Decal Chemicals, it is a 5% formic acid with
> buffers.
> Patsy
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy
> Wenk
> Sent: Friday, July 01, 2005 5:13 PM
> To: 'louise renton'; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] formic acid decal
> 
> A milder formic acid decalcification solution is FASC = formic acid-sodium
> citrate. It's a buffered formic acid. Sections of vertebral bone take 5-7
> days to decalcify. I don't know how long a bone biopsy takes. When my
> students have to submit a bone section for their certification exam, this is
> what we use. It gives great nuclear detail, fantastic eosinophil granules.
> So I expect IHC would be equally great. We use the procedure out of the
> Bancroft book.
> 
> 65 mL of 20% aqueous trisodium citrate (13 g in 65 mL water)
> 35 mL of 90% formic acid (that's full strength. Don't dilute)
> pH is about 2.3
> Change solution every 3 days.
> 
> We did have a rare bone tumor, and none of the IHC worked, until we
> decalcified it in EDTA. Then it worked great. (Sorry, don't remember what
> type of tumor or what type of antibody.) But you might want to try the EDTA,
> if you have a lot of time. Vertebral bone takes 2-3 weeks. The little bone
> fragments we had from the bone tumor took 3 days. (would have been a 2-3
> hours in our usual 5% hydrochloric acid)
> 
> 25 g EDTA disodium salt (ethylenediaminetetracetic acid) (note: MUST be
> disodium salt)
> 175 mL d. water
> Solution is cloudy. Add 2.5-3.0 g of sodium hydroxide until solution becomes
> clear. pH should be about 7 at that point.
> Change solution about twice a week.
> 
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
> 
> 
> Lee & Peggy Wenk
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise
> renton
> Sent: Friday, July 01, 2005 8:32 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] formic acid decal
> 
> Hi all,
> 
> on pain of being repetitive/lazy/slovenly/slothful/unprofessional,
> would someone PLEASE share their recipe for formic acid decalcifying
> solution suitable for samples on which IHc will be performed. Many many
> thanks
> 
> --
> Louise Renton (grovelling)
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> "....I know who I am. No-one else knows who I am. If I was a giraffe, and
> someone said I was a snake, I'd think no, actually I'm a giraffe"
> Richard Gere
> 
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