[Histonet] PCR and ISH in fixed tissues

[Agripina Suarez]

Hi Caroline.
Most of the ISH I do are on  paraformaldehyde/formalin-fixed
paraffin-embedded sections and they work very well. The tissues are
fixed either in 4% paraformaldehyde or in commercially available 10%
neutral buffered formalin for 12 to 24 hours, depends on the size of the
tissue.
Hope this helps.
Agripina


Agripina C. Suarez
McDonald Research Laboratories
iCAPTURE Centre, St Paul's Hospital
1081 Burrard St, Vancouver
B.C., Canada V6Z 1Y6
Tel no. 604 682 2344 local 62562
Fax no. 604 806 8351

>>> Caroline Bass <cbass <@t> bidmc.harvard.edu> 7/4/2005 10:54:08 AM >>>
Hey guys,

My boss is submitting a grant and he does not believe that you can do 
PCR (RT) or ISH with fixed tissues.  I thought you could but I don't 
know any details.  Could someone elaborate?  Is there a particular way

the tissue should be fixed?  In other words do I have to use FFPE 
sections or can I use 4% paraformaldehyde perfusion?

Any advice would be helpful.

Thanks,

Caroline Bass


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