Thank you all for the methods you kindly sent me on Glycine HCl buffer. It is much appreciated. Thanx Melanie. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935