[Histonet] Re: long term storage of slides

LuAnn Anderson ander093 <@t> tc.umn.edu
Wed Jan 5 10:24:29 CST 2005


Bielschowsky silver stain is also affected by long term storage of 
slides--talking months not days. I always cut fresh sections for Biel's.



At 10:06 AM 1/5/05, Gayle Callis wrote:
>Depending on the kind of staining wanted, I have stained paraffin sections 
>on slides that were stored for years and didn't have any adverse 
>effects.  I was not doing immunostaining, just special stains.
>
>Caveat:  some special stains may be affected by long term storage, one 
>reported is Masson's Trichchrome for collagen.  I would also be careful 
>about longer term storage for immunohistochemical stains.  Days are 
>probably not a problem.
>
>This has been discussed before on Histonet, check the archives for further 
>info.
>
>  and At 02:57 PM 1/3/2005, you wrote:
>>Hello everybody,
>>I was wondering how long paraplast sections that have been
>>mounted on slides could be left alone after drying before
>>staining.  Is there any reason they couldn't be left for days
>>or weeks?
>>Thanks for your advice,
>>Frank
>>
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>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>
>
>
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