[Histonet] Re: Gunk in stainer drain line[Scanned]

[Kemlo Rogerson]

PS. I keep fish in a pond outside. In Spring you get a sticky algae/ gunk
that lasts for a few weeks. It's caused by lack of denitrifying bacteria in
the filter (they die over Winter), protein in the water (fish poo and fish
food) and increased light levels and a increasing water temperature (over 10
degrees C). To eradicate you put compacted barley bales into the water which
decompose to produce an organic acid that kills the gunk. You can also buy
Propriety water treatments from the Fish Shop but they only give temporary
relief; once you get balance with a good culture of bacteria in your filter
and sufficient plant growth to remove the nitrates that accumulate from the
breakdown of ammonia by those bacteria, the problem resolves spontaneously.

Does that help?

<g> 

 

-----Original Message-----
From: Marshall Terry Dr, Consultant Histopathologist
[mailto:Terry.Marshall <@t> rothgen.nhs.uk] 
Sent: 17 February 2005 15:38
To: Kemlo Rogerson; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Gunk in stainer drain line[Scanned]

Kemlo,
You believe that (about the breast fat)?

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Kemlo Rogerson [mailto:Kemlo.Rogerson <@t> elht.nhs.uk]
Sent: 17 February 2005 14:17
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Gunk in stainer drain line[Scanned]


When we had these problems we asked the Company about how to solve it. They
asked if we stained breast FNAC's and we did. They suggested it was the fat
from said aspirates; I had always thought it was fungus. We adopted a
protocol of bleach and boiling water and that cured it, but gassed the
BMS's. We had a similar problem in London too, but that was really due to
the sewerage they laughingly call 'drinking water'. The solution was to
connect the stainer to piped 'clean' water; but then no-one drinks the water
in London they all use chillers.

Doesn't explain why the beer 'London pride' is so good, or does it?

Kemlo Rogerson
Cellular Pathology Manager
East Lancashire Hospitals NHS Trust
DD. 01254-294162
Mobile 0774-9754194
 

-----Original Message-----
From: Poteete, Jacquie A. [mailto:japoteete <@t> saintfrancis.com] 
Sent: 17 February 2005 13:35
To: CrochiereSteve <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Gunk in stainer drain line[Scanned]

The "blue goo" in the stainers in our laboratory was found to be a variety
of slime mold. I can't remember its' scientific name, but we had to use
bleach to get rid of it, and the bleach did not prevent it from recurring.
Pouring bleach down the drain became part of our cleaning-maintenance
procedure.  Good luck!

Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa, OK
japoteete <@t> saintfrancis.com

-----Original Message-----
From: CrochiereSteve <@t> aol.com [mailto:CrochiereSteve <@t> aol.com] 
Sent: Wednesday, February 16, 2005 9:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Gunk in stainer drain line


Does anyone have a problem with gunk clogging up the drain line on their 
autostainer. I have a Varistain Gemini, and the line has clogged up with
blue goo 
twice in the past 4 years. What could be the cause and how would I prevent
it, 
short of periodically taking the thing apart and reaming out the hose? 
Pouring bleach down the drain was suggested, but didn't keep the problem
from 
recurring.
Any suggestions? 

Steven M. Crochiere, HT(ASCP)
Histology Supervisor
LifePath Partners @ Mercy Medical Center
Springfield, MA 01104 _______________________________________________
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