[Histonet] Cresyl Violet counterstain

deborah.mills <@t> syngenta.com deborah.mills <@t> syngenta.com
Fri Feb 18 02:21:43 CST 2005

I am attempting to determine dopaminergic cell loss in the SNpc of mouse
brains using a tyrosine hydroxylase antibody. The sections have to be
counterstained with cresyl violet to enable me to count CV+ / TH- neurons in
this region. However, I am having trouble achieving a good enough contrast
between my TH staining (DAB visualisation) and the CV stain to allow me to
count the CV neurons. The CV doesn't appear to be staining up the neurons
very well. Is there anything I can do to improve the intensity of my
staining and be able to pick out the neurons more easily? I have tried
increasing the length of time the sections are in CV solution for but all
this seems to do is increase the background staining without increasing
neuronal staining. I have also tried altering the differentiation steps to
no avail,

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