[Histonet] Marking of mouse brain tissue

deborah.mills <@t> syngenta.com deborah.mills <@t> syngenta.com
Fri Feb 18 02:42:47 CST 2005

I am sectioning Para formaldehyde fixed, sucrose cryoprotected mouse brain
tissue on the microtome to then perform free floating tyrosine hydroxylase
IHC with a cresyl violet counterstain to count CV+/TH- neurons in the SNpc
region.  Before sectioning I need to mark the tissue so I can load them all
onto slides in the same orientation.  I have tried puncturing the tissue
with various sizes of needle and have found it difficult to get a hole large
enough to be visible to the eye yet small enough to avoid the SNpc and avoid
the sections breaking apart during the staining process.  Is there a dye I
could puncture with that wouldn't spread to the rest of the tissue or
interfere with my IHC?  Any other suggestions?

Many thanks in advance.

More information about the Histonet mailing list