[Histonet] antigen retrieval question

Jim Staruk jstaruk <@t> masshistology.com
Thu Feb 17 17:23:52 CST 2005

We tried that once and the control slide (along with the research slides)
were completely negative.  Not sure if it was a fluke, but we never tried it
again!  Now everything is done on the same day.


    Jim Staruk
Mass Histology Service

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Thursday, February 17, 2005 3:33 PM
To: ihcrg <@t> neo.agsci.colostate.edu
Cc: histonet <@t> pathology.swmed.edu
Subject: [Histonet] antigen retrieval question

I have heard that it is not a good idea to do HIER one day and do the IHC
the next?  Is this so?  I heard that the unmasking could actually remask if
left sitting a long time before doing the staining.  I have a protocol that
requires a 3 hr. water bath incubation for AR and it would be more
convienent if I could do that one day and do the rest of the procedure the
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