[Histonet] antigen retrieval question

[Patsy Ruegg]

I have heard that it is not a good idea to do HIER one day and do the IHC
the next?  Is this so?  I heard that the unmasking could actually remask if
left sitting a long time before doing the staining.  I have a protocol that
requires a 3 hr. water bath incubation for AR and it would be more
convienent if I could do that one day and do the rest of the procedure the
next.
Patsy