[Histonet] blue flourescent nuclear dyes

[Barbara Osborn]

Thankyou for your replies.  I found the info that I needed and I apparently
had totally misunderstood the nature of Draq5 and it's excitation and
emmission spectra.  Now it all makes much more sense!  Thanks!

----- Original Message ----- 
From: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>
To: "Barbara Osborn" <bosborn <@t> hsc.usf.edu>; "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 09, 2005 5:32 PM
Subject: RE: [Histonet] blue flourescent nuclear dyes


> Hi Barbara,
>
>
> We use Draq-5 and we get it from Biostatus in the UK. Their website is
> http://www.biostatus.co.uk/.  It is supposed to work in the far-red
> range, but some of our researchers have reported an excitation in 488nm
> and 568 nm as well.  I personally have not witnessed this.
>
> Hope this helps,
> Kathy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barbara
> Osborn
> Sent: Wednesday, February 09, 2005 2:58 PM
> To: Histonet
> Subject: [Histonet] blue flourescent nuclear dyes
>
> We are just beginning flourescent staining for confocal microscopy in
> our lab, and our confocal microscope did not come with a UV laser, so we
> cannot detect DAPI as a nuclear stain.  We've been told to use Draque 5
> (sp) which emits blue in visible light instead of DAPI to counterstain
> nuclei.  I cannot for the life of me find any info on this dye.
> Molecular Probes offers a blue flourescent nuclear dye called BOBO-1
> iodide.  Does anyone out there in histoland have any information about
> Draque 5, where to purchase it,  or has anyone tried the BOBO-1 from
> Moleculare Probes?  Or does anyone use an alternative nuclear dye?  We
> plan to triple stain using nuclear dye/ Alexa Flour 488/ Alexa Flour 568
> so I need something that does not overlap too much with the 488.
> Thanks.
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