[Histonet] blue flourescent nuclear dyes
bosborn <@t> hsc.usf.edu
Thu Feb 10 08:52:17 CST 2005
Thankyou for your replies. I found the info that I needed and I apparently
had totally misunderstood the nature of Draq5 and it's excitation and
emmission spectra. Now it all makes much more sense! Thanks!
----- Original Message -----
From: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>
To: "Barbara Osborn" <bosborn <@t> hsc.usf.edu>; "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 09, 2005 5:32 PM
Subject: RE: [Histonet] blue flourescent nuclear dyes
> Hi Barbara,
> We use Draq-5 and we get it from Biostatus in the UK. Their website is
> http://www.biostatus.co.uk/. It is supposed to work in the far-red
> range, but some of our researchers have reported an excitation in 488nm
> and 568 nm as well. I personally have not witnessed this.
> Hope this helps,
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barbara
> Sent: Wednesday, February 09, 2005 2:58 PM
> To: Histonet
> Subject: [Histonet] blue flourescent nuclear dyes
> We are just beginning flourescent staining for confocal microscopy in
> our lab, and our confocal microscope did not come with a UV laser, so we
> cannot detect DAPI as a nuclear stain. We've been told to use Draque 5
> (sp) which emits blue in visible light instead of DAPI to counterstain
> nuclei. I cannot for the life of me find any info on this dye.
> Molecular Probes offers a blue flourescent nuclear dye called BOBO-1
> iodide. Does anyone out there in histoland have any information about
> Draque 5, where to purchase it, or has anyone tried the BOBO-1 from
> Moleculare Probes? Or does anyone use an alternative nuclear dye? We
> plan to triple stain using nuclear dye/ Alexa Flour 488/ Alexa Flour 568
> so I need something that does not overlap too much with the 488.
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> Histonet <@t> lists.utsouthwestern.edu
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