[Histonet] blue flourescent nuclear dyes

[John Kiernan]

If you want a blue emission you'll almost certainly
need near-UV excitation. The excitation wavelength
of a fluorochrome is always shorter than the wavelength
of the emitted light. 
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Barbara Osborn wrote:
> 
> We are just beginning flourescent staining for confocal microscopy in our lab, and our confocal microscope did not come with a UV laser, so we cannot detect DAPI as a nuclear stain.  We've been told to use Draque 5 (sp) which emits blue in visible light instead of DAPI to counterstain nuclei.  I cannot for the life of me find any info on this dye.  Molecular Probes offers a blue flourescent nuclear dye called BOBO-1 iodide.  Does anyone out there in histoland have any information about Draque 5, where to purchase it,  or has anyone tried the BOBO-1 from Moleculare Probes?  Or does anyone use an alternative nuclear dye?  We plan to triple stain using nuclear dye/ Alexa Flour 488/ Alexa Flour 568 so I need something that does not overlap too much with the 488.  Thanks.
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