[Histonet] blue flourescent nuclear dyes

[Walters, Katherine S]

Hi Barbara,


We use Draq-5 and we get it from Biostatus in the UK. Their website is
http://www.biostatus.co.uk/.  It is supposed to work in the far-red
range, but some of our researchers have reported an excitation in 488nm
and 568 nm as well.  I personally have not witnessed this.  

Hope this helps,
Kathy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barbara
Osborn
Sent: Wednesday, February 09, 2005 2:58 PM
To: Histonet
Subject: [Histonet] blue flourescent nuclear dyes

We are just beginning flourescent staining for confocal microscopy in
our lab, and our confocal microscope did not come with a UV laser, so we
cannot detect DAPI as a nuclear stain.  We've been told to use Draque 5
(sp) which emits blue in visible light instead of DAPI to counterstain
nuclei.  I cannot for the life of me find any info on this dye.
Molecular Probes offers a blue flourescent nuclear dye called BOBO-1
iodide.  Does anyone out there in histoland have any information about
Draque 5, where to purchase it,  or has anyone tried the BOBO-1 from
Moleculare Probes?  Or does anyone use an alternative nuclear dye?  We
plan to triple stain using nuclear dye/ Alexa Flour 488/ Alexa Flour 568
so I need something that does not overlap too much with the 488.
Thanks.
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