[Histonet] CD-20 nuclear staining....
katri <@t> cogeco.ca
Wed Feb 2 15:51:10 CST 2005
I have seen this occasionally with various antibodies. It seems to be a
combination of too short a fixation and HIER. Properly fixed control
material does not show this false positivity.
Hamilton, Ontario, Canada
----- Original Message -----
From: "Brandon Stokes" <cuttingedgehistology <@t> yahoo.com>
To: <Histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, February 01, 2005 9:26 PM
Subject: [Histonet] CD-20 nuclear staining....
> Hello fellow histo-netters,
> I was wondering if anyone else has ever experienced nuclear staining
> occuring when using the CD-20 antibody. The tonsil control will look
> great....then you look at the patient tissue(almost always skin for us)
> and some of the nuclei of the keratinocytes in the epidermis and even some
> nuclei in the dermis might light up bright red(we use AEC). You can
> actually see the nuclear detail very well. This problem doesn't happen
> all the time so I am not sure what it could be.
> Any advice would be greatly appreciated.
> Brandon Stokes, HT(ASCP)
> Lab Manager
> Cutting Edge Histology Services, LLC
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