[Histonet] CD-20 nuclear staining....

Brandon Stokes cuttingedgehistology <@t> yahoo.com
Tue Feb 1 20:26:18 CST 2005

Hello fellow histo-netters,
I was wondering if anyone else has ever experienced nuclear staining occuring when using the CD-20 antibody.  The tonsil control will look great....then you look at the patient tissue(almost always skin for us) and some of the nuclei of the keratinocytes in the epidermis and even some nuclei in the dermis might light up bright red(we use AEC).  You can actually see the nuclear detail very well.  This problem doesn't happen all the time so I am not sure what it could be.  
Any advice would be greatly appreciated.
Brandon Stokes, HT(ASCP)
Lab Manager
Cutting Edge Histology Services, LLC

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