[Histonet] problem with autoflurescence

[Ali Moussavi Nik]

Dear All

Happy new year

I am doing Immunohistochemistry on 4% PFA fixed, vibrotome sectioned
embryonic brain. My antibody is FITC conjugated  

I have a problem with the background green Autoflorescence of the PFA .

Can anyone propose me other method of fixation for embryonic brain.

Thanks in advance.

Best wishes for the new year.

Ali

 

 

 

 

 

 

 

 

Seyed Ali Moussavi Nik D.V.M , PhD Student

Department of Cell and Molecular Biology, 

Gothenburg University

Box 462 

S-405 30 Göteborg 

Sweden

Phone: +46(031) 7733895

Fax:     +46(031) 7733801

 <http://www.molbio.gu.se> www.molbio.gu.se