[Histonet] problem with autoflurescence
Ali Moussavi Nik
ali.moussavi <@t> molbio.gu.se
Fri Dec 30 04:15:32 CST 2005
Happy new year
I am doing Immunohistochemistry on 4% PFA fixed, vibrotome sectioned
embryonic brain. My antibody is FITC conjugated
I have a problem with the background green Autoflorescence of the PFA .
Can anyone propose me other method of fixation for embryonic brain.
Thanks in advance.
Best wishes for the new year.
Seyed Ali Moussavi Nik D.V.M , PhD Student
Department of Cell and Molecular Biology,
S-405 30 Göteborg
Phone: +46(031) 7733895
Fax: +46(031) 7733801
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