[Histonet] Re:Sections coming off!

Shannon Bryce Shannon.Bryce <@t> UTSouthwestern.edu
Tue Dec 20 13:01:17 CST 2005


We get our slides which are the + coated slides from Fisher.  Our MMA
sections are also falling off here lately so I went to another
department and borrowed a box of theirs that they bought through VWR and
have had no problems so I would guess it's a bad lot.....anyone have lot
numbers?

Thanks,
Shannon Bryce 
UT Southwestern Medical Center

>>> "Joanne Mauger" <mauger <@t> email.chop.edu> 12/20/05 8:39 AM >>>
Hi Patricia,

Are you using slides from Erie? We buy ours through Fisher, and have
been having trouble keeping sections on + slides as well. It is as if
they have no charge at all. We think the lot may be bad. The company
claims to have no complaints.

Anyone else having similar problem?
Jo Mauger 

>>> "Patricia F Lott" <plott <@t> uab.edu> 12/19/05 3:21 PM >>>
Help!  We are losing our thin plastic sections!  We have cut PMMA
plastic sections at 5 microns and put them on "+" slides, and the
sections fall off either during de-plasticizing or during staining! 
We
have tried:  Haupt's adhesive, longer drying times, "+" slides, plus
slides and Haupt's together, etc....no luck!  Any suggestions would be
welcome!
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